大豆皂甙Bb对食管癌细胞凋亡的影响及其机制研究

目的探讨大豆皂甙Bb(SSBb)抑制人食管癌细胞Eca-9706生长、诱导凋亡的效应及其机制。方法用噻唑蓝比色法测不同浓度SSBb对Eca-9706细胞生长的抑制率;缺口末端标记法检测Eca-9706细胞的凋亡率;免疫组织化学染色法和免疫印迹法检测人食管癌Eca-9706细胞在SSBb作用下c-met、VEGF、cyclinD1、NF-κB、PTEN、HDAC1、caspase3蛋白表达的变化。结果与对照组相比,SSBb处理的Eca-9706细胞的生长抑制率、凋亡率增加,caspase3、PTEN的免疫反应性和印迹信号增强,cyclinD1、c-met、VEGF、NF-κB、HDAC1的免疫反应性和印迹信号减弱(P0.01)。结论 SSBb对人食管癌Eca-9706细胞增殖有抑制作用,可降低Eca-9706细胞中的c-met和VEGF恶性标志的过高表达,并通过抑制HDAC1、NF-κB,激活PTEN和caspase3信号传导途径诱导食管癌细胞凋亡。

Apoptosis effects on human esophageal cancer cells by soyasaponin Bb and its machanism

Objective To explore the effect and potential mechanism of apoptosis in the Eca-9706 cells induced by soyasaponin Bb.Methods Different concentrations of soyasaponin Bb were on the Eca-9706 cell.Cell growth suppression rate was detected by MTT.Apoptotic rate was detected by TUNEL.The expression changes of HDAC1, NF-κB,PTEN,cyclin D1,c-met,VEGF or caspase-3 in the SSBb were detected by immunohistochemistry and immunoblotting.Results Compared with the control group,the growth-inhibition rate and apoptotic rate were increased,the expressions of PTEN,caspase 3 were increased,the expressions of c-met,VEGF,HDAC1,NF-κB and cyclin D1 were decreased.Conclusion SSBb can suppress Eca-9706 cell growth.SSBb can exhibit reverse effects on over expression of c-met,VEGF in Eca-9706 cells.Eca-9706 cell apoptosis can be induced by SSBb through inhibiting HDAC1-NF-κB and activating PETEN and caspase-3 signaling pathways.