甲醛致胎鼠肝微核率及染色体畸变的研究

目的探讨甲醛致胎鼠肝微核率与染色体畸变。方法随机将妊娠小鼠(孕13天)分为5组:腹腔注射甲醛(0.00、0.20、2.00、20.00mg/kg)染毒组、环磷酰胺(30mg/kg)阳性对照组,实验至妊娠第14天即染毒后18小时脱颈椎处死孕鼠,剥离两侧子宫,每侧各取一胎鼠,断头放出外周血,取胎肝,采用胎肝微核试验和胎肝染色体畸变试验,检测胎肝血微核率和染色体畸变率。结果2.00、20.00mg/kg甲醛染毒组胎肝微核率及胎肝染色体畸变率与阴性对照组比较差异有显著性(P<0.01);染色体畸变主要表现为染色体断裂、多倍体等畸形。结论甲醛可致胎鼠肝微核率与染色体畸变。

Micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde

Objective To study the micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde. Methods The pregnant mice (been pregnant for 13 days) were divided into 5 groups randomly: Formaldehyde was given as intraperitoneal injection (0.00, 0.20, 2.00, 20.00mg/kg) for exposure group and Cyclophosphamide (30mg/kg) for positive control group. When the 14th days (I.e After exposed in formaldehyde 18 hours), escapes the cervical vertebra cervical killed off pregnant mouse, peels on both sides of the uterus, takes an embryo respectively each side, beheads and emits the circumference blood, takes out the embryo liver, performs micronucleus experiment and chromosome aberration experiment by use of embryo liver, validate embryo liver blood micronucleus rate and the chromosome distortion factor. Results The micronucleus rate and chromosome aberration (mainly for chromosome breakage 、 polyploid ) rate of embryonic liver showed significant differences in formaldehyde exposure group (2.00, 20.00mg/kg) compared with the control group ( P < 0.01 ). Conclusion The micronucleus rate and chromosome aberration of mouse embyos liver induced by formaldehyde .