汉族人群Trib1同源基因rs17321515多态性分布及其与血脂水平相关性研究

目的检测Trib1同源基因rs17321515位点基因型多态性分布状况,并探索该位点多态性与不同血脂指标和血脂临床诊断结果的关系。方法采集1 014名体检者的血样,进行血脂四项指标测定,并采用MALDI-TOF质谱技术对rs17321515位点基因型进行分型检测。结果 rs17321515位点基因型构成比为:A/A∶A/G∶G/G=13.8%∶50.2%∶36.0%,等位基因频率为A∶G=38.9%∶61.1%。方差分析中,男性不同基因型的TG水平存在统计学差异(F=4.46,P=0.01),AG携带者比GG携带者的TG水平低(t=-0.29,P=0.02);Logistic回归分析表明,男性AA型携带者高TG血症患病率低于GG型(OR=0.45,P=0.04),男性AG型携带者低HDL-c血症患病率低于GG型携带者(OR=0.62,P=0.03),女性AG型携带者高TC血症患病率低于GG型携带者(OR=0.58,P=0.01)。结论 rs17321515位点等位基因频率在不同种族人群存在一定差异;该位点多态性对多个血脂指标和血脂异常临床诊断都有影响,但这种影响存在性别差异;A等位基因是血脂水平的保护因素。

SNP of rs17321515 homologous with Trib1 in Han population and its correlation with blood lipid

Objective To study on genotype distribution of rs17321515 which is homologous with Trib1 and its associativity with blood lipid parameters and diagnosis.Method Collecting 1 014 blood samples to measure its lipid levels,then detecting rs17321515 locus's SNP by MALDI-TOF mass spectrometry technology.Results three genotypes(A/A,A/G,G/G) proportions in the population were 13.8%,50.2% and 36.0%,allele frequencies were A∶G=38.9%∶61.1%.In variance analysis,variation of TG of male was statistical significant(F=4.46,P=0.01),TG level of male who carried AG is lower than GG carries(t=0.29,P=0.02).Logistic regression analysis showed that,hypertriglyceridemia prevalence of male who carried AA is lower than GG carries(OR=0.45,P=0.04).Low HDL-c acidosis prevalence of male who carried AG is lower than GG carries(OR=0.62,P=0.03).Hypercholesterolemia prevalence of female who carried AG is lower than GG carries(OR=0.58,P=0.01).Conclusion Allele frequencies of rs17321515 varies in different ethnic groups;The locus' polymorphism distribution is relevant with a certain lipid indicators and lipid diagnosis,but there exist gender differences on these relevance.