细颗粒物污染及对炎性因子IL-6表达的影响

为比较细颗粒物污染水平及研究其炎性损伤毒性 ,选取我国两个不同污染特征的城市太原与北京(燃煤污染 ,燃煤与汽车尾气混合污染 ) ,采用分级采样器收集两城市空气中细颗粒物样品 ,用重量法分析细颗粒物质量浓度 ,细颗粒物上的B(a)P用甲醇超声提取 ,Pb用硝酸和过氧化氢提取 ,比较分析了两城市细颗粒物污染水平 ;同时用ELISA及RT PCR法 ,以人肺泡上皮细胞 (A5 49)作为靶细胞 ,测定了细颗粒物对上皮细胞产生的炎性因子IL 6表达的影响。结果显示 ,以美国EPA大气环境质量PM2 5 标准为参考 ,PM2 5 太原冬季、北京冬季超标率、超标倍数分别为 10 0 % ,4 2 3 ;90 6% ,2 62。太原冬季、北京冬季空气中B(a)P浓度分别为58 6和 10 9ng m3 ,均超过我国标准。北京、太原空气颗粒物上Pb含量均未超过我国居住区大气Pb标准。细颗粒物能引起人肺上皮细胞产生炎性因子IL 6及其mRNA的表达增加 ,而且呈现剂量 -效应关系。本次研究地区空气细颗粒物污染比较严重 ,并具有一定程度的炎性损伤毒性

Fine particles pollution and its impact on the expression of inflammation cytokine IL-6

To investigate fine particles (PM2.5) pollution and its impact on the expression of inflammation cytokine IL-6. Two cities, namely Taiyuan and Beijing, were selected to represent two different types of air pollution. Classification air sampler was used for PM2.5 collection in the air, and weighting method was used to analyze PM2.5 mass concentration. B (a) P in PM2.5 was extracted by methyl alcohol using ultrasonic method and Pb in PM2.5 was dissolved in nitric acid and hydrogen peroxide. Human alveolar epithelial cell line (A549) was used as target cell. Concentration of inflammation cytokine IL-6 was measured by an ELISA kit and, the mRNA expression of IL-6 was measured by RT-PCR while A549 cells were incubated with PM2.5 in different doses. Air Quality Standard for PM2.5 of U S EPA was applied as the reference. The percentage and multiple of exceeding standard of PM2.5 concentration of Taiyuan winter, Beijing winter and Beijing spring were 100%, 4.23; 90.6%, 2.62; 97.4%, 2.53 respectively. B(a) P in Taiyuan winter and Beijing winter were 5.86, 1.09 micrograms/100 m3) respectively that was over the standard. Pb level of particle matter in two cities was under the Air Guality Standard of Residential Area in China. It was showed an increase of protein concentration and mRNA expression of IL-6 in A549 cultured with PM2.5 compared with untreated cells, presenting a dose-effect relationship. It is suggested that PM2.5 pollution was relatively heavy in the study areas and showed inflammation damage toxicity.