双酚A对植入后大鼠、小鼠胚胎致畸性的体外实验研究

为揭示双酚A(BPA)的发育毒性及其可能的作用机制 ,采用植入后全胚胎培养模型 ,将 8 5日龄BalB C小鼠胚胎和 9 5日龄大鼠胚胎分别与含BPA的大鼠即刻离心血清共培养 48h(BPA终浓度为 0、40、60、80、10 0mg L) ,观察双酚A对小鼠和大鼠胚胎生长发育和组织器官形态分化的影响。结果显示 ,随着BPA剂量的增加胚胎在体外的生长发育受影响越严重 ,呈现出明显的剂量 -效应及剂量 -反应关系。≥ 60mg L的BPA可诱发小鼠及大鼠胚胎的卵黄囊生长和血管分化不良、生长迟缓及形态分化异常 ,严重者出现体位异常、神经系统、鳃弓发育异常及小眼、小肢芽等畸形。提示在较高剂量下 ,BPA对体外培养的大鼠和小鼠胚胎具有一定的毒性作用 ,其中对卵黄囊胎盘的结构和功能的影响可能是其致畸作用的机制之一

Teratogenicity of bisphenol A on post-implanted rat and mouse embryos:an in vitro study

In order to evaluate the developmental toxicity of BPA and to investigate the possible mechanism of it in vitro, whole embryo culture was used for the study. The embryos that removed from mother mice on gestational 8.5d (GD8.5) and mother rats on gestational 9.5d (GD9.5) were cultured immediately in centrifugal serum (ICS) with a series of concentrations of 0, 40, 60, 80, 100 mg/L of BPA for 48 h to evaluate the embryo development and morphological differentiation. The results showed that the BPA inhibited the development of embryos in vitro and there was a dose-response relationship between concentrations of BPA. At high level (> or = 60 mg/L), BPA could delayed the growth and cardiac tube differentiation of visceral yolk sac (VYS), and induced various embryo defects, including abnormal neural system, arch, optic, flexion and small limb bud, etc. It is concluded that at high level, BPA had toxicity to mice and rat embryos in vitro. The damage on structure and function of VYS might be part of teratogenic mechanisms of BPA.