饮水氯化消毒副产物MX对人胚肝细胞(L-02)的DNA损伤及凋亡作用

目的　研究饮水氯化消毒副产物 3 氯 4 二氯甲基 5 羟基 2 (5氢 ) 呋喃酮 (MX)致人胚肝细胞 (L 0 2 )DNA损伤及凋亡作用。方法　以L 0 2为靶细胞 ,采用单细胞凝胶电泳技术 (SCGE)和流式细胞术 (FCM)分别检测MX致L 0 2细胞DNA损伤及凋亡作用。结果　随着MX浓度的增加 ,L 0 2细胞DNA链断裂逐渐增加 ,且 10 0和 30 0 μmol L剂量组与溶剂对照组相比具有统计学显著性差异 (P <0 0 5 ,P <0 0 1) ;MX各剂量组均引起L 0 2细胞凋亡率明显增加 ,与溶剂对照组相比具有统计学显著性差异 (P <0 0 0 1)。结论　饮水氯化消毒副产物MX可致L 0 2细胞DNA单链断裂和凋亡

DNA damage and apoptosis in L-02 cells induced by chlorination by-product MX of drinking water

OBJECTIVE: To study DNA damage and apoptosis induced by chlorination by-product MX of drinking water. METHODS: Single cell gel electrophoresis (SCGE) and flow cytometry were used to detect DNA damage and apoptosis in human fetal hepatocytes (L-02) treated with MX. RESULTS: DNA strand breaks were found to increase in L-02 cells treated with MX in a dose-response manner. On treatment levels of 100 and 300 micromol/L, MX led to significant increase of DNA damage in comparison to the solvent controls (DMSO) (P < 0.05, and P < 0.01 respectively). Statistically significant increase of L-02 cell apoptosis were observed in all MX treated groups in comparison to the solvent controls (DMSO) (P < 0.001). CONCLUSION: Chlorination by-product MX of drinking water could induce obviously DNA damage and apoptosis in L-02 cells.