黄芩提取物联合顺铂对人卵巢癌细胞株SKOV-3凋亡的研究

目的:探讨黄芩提取物(Scutellaria Baicalensis)与顺铂(cisplatin,DDP)联合应用对卵巢癌细胞系SKOV-3增殖及凋亡的影响。方法:采用MTT比色法检测S.Baicalensis与DDP联合应用对SKOV-3细胞增殖的影响,吖啶橙染色观察诱导细胞凋亡情况,流式细胞仪分析细胞周期及凋亡并计算细胞平均凋亡率,Westernblot技术观察细胞内p53、ERK1/2、p-STAT3的表达。结果:300μg/mlS.Baicalensis联合5μg/ml DDP给药:①48h后可使肿瘤细胞抑制率由单独应用DDP的15.8%提高到37.3%;②吖啶橙荧光染色可见细胞呈现明显凋亡形态,其凋亡率与10μg/ml DDP组相当;③流式细胞检测结果显示,可使细胞凋亡率从15.1%提高到42.2%(P<0.05);④westernblot结果显示,p53、ERK1/2、p-STAT3表达较5μg/ml DDP组单独给药组降低(P<0.05)。结论:S.Baicalensis与DDP联合应用增强对卵巢癌细胞株SKOV-3的致凋亡效应,联合应用可达到减毒增效的效果。

Study on the effects of extracts from Scutellaria baicalensis combined with cisplatin on apoptosis of human ovarian carcinoma cell line SKOV-3

Objective:To explore the effects of extracts from Scutellaria baicalensis combined with cisplatin on proliferation and apoptosis of human ovarian carcinoma cell line SKOV-3.Methods:MTT colorimetry was used to detect the effects of extracts from Scutellaria baicalensis combined with cisplatin on proliferation of human ovarian carcinoma cell line SKOV-3;acridine orange staining was used to observe the apoptosis of human ovarian carcinoma cell line SKOV-3;flow cytometry was used to analyze cell cycle and apoptosis;the average apoptosis rate was calculated;Western blot was used to observe the expressions of p53,ERK1/2 and p-STAT3.Results:After treated with 300μg/ml extracts from Scutellaria baicalensis and 5μg/ml cisplatin for 48 hours,the inhibition rate of human ovarian carcinoma cell line SKOV-3 increased from 15.8%(treated with cisplatin only) to 37.3%;acridine orange staining showed apoptotic cells,the apoptosis rate was similar to that of 10μg/ml cisplatin group;the results of flow cytometry showed that the apoptosis rate increased from 15.1% to 42.2%(P<0.05);the results of Western blot showed that the expression levels of p53,ERK1/2 and p-STAT3 were significantly lower than those in 5μg/ml cisplatin group(P<0.05).Conclusion:The extracts from Scutellaria baicalensis combined with cisplatin can enhance the apoptotic effect on human ovarian carcinoma cell line SKOV-3,reduce toxicity and improve clinical efficacy.