卵巢癌异位人绒毛膜促性腺激素的表达研究

目的:研究人绒毛膜促性腺激素β亚基(β-HCG)在卵巢癌组织中的表达特点,探讨异位β-HCG与卵巢癌组织类型、分化程度之间的关系。方法:选择2006年11月～2011年11月在吉林大学第二医院妇产科住院的64例患者的手术切除标本,其中正常卵巢组织(对照组)10例、卵巢良性肿瘤组织(良性组)15例、卵巢交界性肿瘤组织(交界组)13例、卵巢癌组织(恶性组)26例。根据细胞分化程度不同,恶性组又分为低分化组(13例)、中分化组(7例)及高分化组(6例)。应用免疫组织化学法检测3组β-HCG的表达水平。结果:β-HCG在正常对照组、卵巢良性肿瘤组与卵巢交界性肿瘤组中均无阳性表达,异位β-HCG在卵巢上皮癌中的阳性表达率高达53.8%;高分化组1例阳性,阳性率为16.7%;中分化组3例阳性,阳性率为42.9%;低分化组10例阳性,阳性率76.9%,差异有统计学意义(P<0.05)。结论:异位β-HCG的表达与卵巢恶性肿瘤发生相关,异位β-HCG的阳性表达率与肿瘤分化程度关系密切,分化愈差的癌细胞异位β-HCG的表达率愈高,表达强度愈强,异位β-HCG有望作为新的卵巢肿瘤标志物来帮助判断肿瘤的良恶性及治疗后的病情转归。

Study on the expression of ectopic human chorionic gonadotropin in ovarian cancer

Objective:To research the characteristics of expression of β-human chorionic gonadotropin(β-HCG) in ovarian cancer tissue,explore the relationship between ectopic β-HCG and histological types and differentiation degrees of ovarian cancer. Methods:The resected samples of 64 patients who hospitalized in the hospital from November 2006 to November 2011 were selected,including 10 patients with normal ovarian tissue(control group),15 patients with benign ovarian tumor tissue(benign group),13 patients with boundary ovarian tumor tissue(boundary group),and 26 patients with ovarian cancer tissue(malignant group).According to the degrees of differentiation,the patients in malignant group were divided into low differentiation group(13 patients),moderate differentiation group(7 patients),and high differentiation group(6 patients).Immunohistochemical method was used to detect the expressions of β-HCG in the three groups. Results:β-HCG didn’t expressed in control group,boundary group,and malignant group;the positive expression rate of ectopic β-HCG in epithelial ovarian cancer was as high as 53.8%.The positive expression rates of ectopic β-HCG in high differentiation group,moderate differentiation group,and low differentiation group were 16.7%(one patient),42.9%(three patients),and 76.9%(ten patients),respectively,there was statistically significant difference among the three groups(P<0.05). Conclusion:The expression of ectopic β-HCG is correlated with the occurrence of ovarian malignant tumor,the positive expression rate of ectopic β-HCG is closely related to the degrees of differentiation,the poorer the differentiation is,the higher the expression rate of ectopic β-HCG and expression intensity are;ectopic β-HCG may be used as a new tumor marker to predict the tumor nature and prognosis after treatment.