孕早期胎盘HBV感染的危险因素分析

目的:观察孕早期HBsAg阳性孕妇流产胎盘组织(乙型肝炎病毒)HBV感染情况,分析可能的危险因素,为进一步探讨HBV宫内感染机制提供线索,并为预防措施的制订提供初步的理论依据。方法:采用巢式病例对照研究,对355例自愿流产孕妇中外周血HBsAg阳性者按统一的调查表进行调查,并对其流产胎盘进行免疫组化和HBVDNA原位杂交检测,运用SPSS10.0统计软件对资料和结果进行统计处理和分析。结果:孕妇HBsAg携带率为7%;早期发育的胎盘HBV感染率为32%;孕妇外周血HBVDNA的高浓度(OR=22.5,P=0.004)和HBeAg阳性(OR=12.5,P=0.008),是孕早期胎盘感染的主要危险因素。结论:HBV可感染早期发育的胎盘,感染率为32%;血HBVDNA的高浓度和HBeAg阳性的孕妇,其孕早期胎盘感染HBV的可能性较大。

Analysis on risk factors of HBV infection in aborted first-trimester placenta

Objective:To observe the presence of HBV in aborted first-trimester placental cells from HBsAg carrying pregnant women and to trace the risk factors of intrauterine infection and the cellular mechanism of HBV transmitting.Methods:ELISA,PCR,SABC immunohistochemical staining with monoclonal anti-HBs,anti-HBc antibodyies and in situ hybridization were used for detection of HBV infection markers in placenta.The epidemiological data were managed and analyzed by SPSS 10.0.Results:The detected positive rate of HBsAg was 7%(25/355) in pregnant women.HBsAg were detected in 8 of 25 placentas of HBsAg positive pregnant women.High HBV DNA concentration and HBeAg positive in serum of pregnant women was the risk factors of intrauterine infection.Conclusion:HBV may infect in first-trimester pregnant placenta with 32%;Pregnant women with high HBV DNA concertration and positive HBeAg who have more possibilities of HBV infection in aborted first-trimester placenta.