人宫颈癌干细胞的分离鉴定

目的:通过分选人宫颈癌细胞中的侧群细胞验证其肿瘤干细胞特性。方法:取16例行宫颈鳞癌切除的手术标本进行宫颈癌细胞原代培养,流式细胞仪分选侧群细胞(Side Population,SP),对比SP细胞和非SP细胞(Non Side Population,Non-SP)的体外自我更新能力和肿瘤形成能力。结果:在原代培养的16株宫颈癌细胞中均分选出SP细胞,比例约占(1.67±0.94)%。SP细胞的克隆形成能力显著高于NSP细胞,SP细胞在裸鼠肿瘤形成能力显著高于NSP细胞。结论:人宫颈癌侧群细胞具有肿瘤干细胞特性,为宫颈癌干细胞的研究提供了简单、有效的分离方法。

Isolation and identification of human cervical cancer stem cells

Objective:To identify the characteristics of cancer stem cells by isolating side population(SP) cells from human cervical cancer cells.Methods:Sixteen surgical samples of cervical squamous cell carcinoma were selected to culture passage cervical cancer cells,flow-cytometry was used to isolate SP cells;the in vitro self-renewal abilities and tumorigenic abilities of SP cells and non-SP cells were compared.Results:SP cells were isolated from 16 cervical cancer cell line after passage culture,accounting for(1.67±0.94)%.The clone formation ability of SP cells was significantly higher than that of non-SP cells,the tumorigenic ability of SP cells in nude mice was significantly higher than that of non-SP cells.Conclusion:Human cervical cancer SP cells of have the characters of cancer stem cells,which provides a simple and valid method for research of cervical cancer stem cells.