肝癌及癌旁肝组织的PCNA免疫组化研究

对３９例肝细胞癌（简称肝癌）及其癌旁肝组织作ＰＣＮＡ免疫组化研究，结果显示：癌与癌旁ＰＣＮＡ阳性率有非常显著差异，癌的阳性率为２５．５％，而癌旁仅１％。肝癌分化越差，ＰＣＮＡ阳性率越高。癌旁以小细胞性肝细胞异型增生阳性率较高，其阳性率与紧邻肝组织的阳性率有显著差异。肝癌ＰＣＮＡ阳性细胞数与核分开数之比为２１：１。肝癌ＰＣＮＡ阳性细胞数不成比例地多于核分裂数，认为可能是由于癌细胞ＤＮＡ内复制所致。因

IMMUNOHISTOCHEMICAL STUDY OF PCNA IN HEPATOCELLULAR CARCIN0MA AND THEIR PERICARCINOMA TISSUES

Thirty nine cases of hepatocellular carcinoma(HCC)with their pericarcinomous tissues(PT) were studied immunohistochemically by using monoclonal antibodies PC10 to proliferating cell nuclearantigen(PCNA).The results showed that the PCNA positive rate of HCCs was very significantly higherthan that of PT,The lower the differentiation of HCC,the higher the PCNA positive rate. Among variouslesions of PT,small cell liver cell dysplasia was the highest one in PCNA positivity, The ratio of PCNApositivity to mototic figures in HCCs was 21: 1,but in erythroblasts of fetal liver(controls),it was 11:1.The latter was more approximate to the theoretical value,We suggested that this was due to endoreduplica-tion fo DNA in HCC cells.So,PCNA might be of practical use on the evaluation of the proliferation ofHCC.