癌基因c-Src抑制剂对三阴性乳腺癌的疗效评价 |
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引用本文: | 周士波,;姚宇锋.癌基因c-Src抑制剂对三阴性乳腺癌的疗效评价[J].中国肿瘤外科杂志,2014(6):359-362. |
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作者姓名: | 周士波 ;姚宇锋 |
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作者单位: | [1]江苏省肿瘤医院CT室,江苏南京210009; [2]江苏省肿瘤医院普外科,江苏南京210009; |
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基金项目: | 江苏省自然科学基金项目(No.BK20131016) |
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摘 要: | 目的:探讨癌基因C-Src抑制剂对三阴性乳腺癌的辅助治疗作用。方法体外培养三阴性乳腺癌细胞 MDA-MB-231,并与雌激素受体(ER)、孕激素受体(PR)阳性的乳腺癌细胞 T47D 以及HER2阳性的SK-Br-3细胞做比较。用癌基因 c-Src 抑制剂来治疗三株细胞。采用 MTT 和免疫电泳的方法检测细胞的生长以及细胞信号传导通路的改变。结果c-Src抑制剂可以部分抑制 T47D 细胞生长,对三阴性乳腺癌细胞 MDA-MB-231有很明显的抑制作用。但是,对 SK-Br-3细胞的生长没有阻滞作用。进一步研究发现,是否抑制细胞生长信号传导通路决定 c-Src 抑制剂的治疗效果。HER2抑制剂可以明显抑制 SK-Br-3细胞周期与生长,而对 MDA-MB-231没有效果。结论抑制癌基因c-Src酪氨酸激酶活性对三阴性乳腺癌细胞有很好的治疗效果。
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关 键 词: | 三阴性乳腺癌 c-Src 基因 c-Src 抑制剂 基因治疗 |
Discussion of the therapeutic effects of oncogene c-Src inhibitor on the triple negative breast cancer |
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Institution: | ZHO U Shibo , YA O Yufeng ( 1. Department of CT room, Jiangsu Cancer Hospital , Nanjing 210009, China ;2. Department of General Surgery, Jiangsu Cancer Hospital, Nanfing 210009, China ) |
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Abstract: | Objective To explore adjuvant therapy on triple negative breast cancer (TNBC).Methods Develop triple negative breast cancer cell MDA-MB-231 cells,comparing with estrogen receptor (ER)and pro-gesterone receptor (PR)positive T47D cells,and HER2 positive Sk-Br-3 cells.They were treated with the in-hibitor of oncogene c-Src.MTT assay was used to measure cell growth.MTT and immunoelectrophoresis were performed to detect the growth of cells and the change of its signaling pathways.Results The c-Src inhibitor partially inhibited cell growth in T47D.It significantly abolished cell proliferation in triple negative MDA-MB-231 cells.However,it has no inhibitory effect on Sk-Br-3 cells.It was found that therapeutic effects of the c-Src inhibitor were related with the blocking cell growth signaling pathways in different cell lines.The HER2 in-hibitor significantly blocked S phase of cell cycles and cell growth in Sk-Br-3 cells,without any effect on MDA-MB-231 cells.Conclusions Inhibition of the tyrosine kinase activity of c-Src is an effective therapy to treat tri-ple negative breast cancer cells. |
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Keywords: | triple negative breast cancer oncogene c-Src c-Src inhibitor gene therapy |
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