卵巢粘液性肿瘤P53蛋白的表达及与DNA·论著·含量关系的研究

目的研究卵巢粘液性肿瘤中Ｐ５３蛋白的表达及与病理组织学分型，分级，临床分期，ＤＮＡ含量的关系。方法应用免疫组织化学及图像分析技术对６５例各种组织学类型卵巢粘液性肿瘤中Ｐ５３蛋白的表达及ＤＮＡ倍体进行了观察。结果Ｐ５３蛋白表达率在囊腺瘤为阴性，交界性囊腺瘤为１７．４％，囊腺癌为５０．０％，囊腺癌的表达率显著高于囊腺瘤（Ｐ＜０．００１）和交界瘤（Ｐ＜０．０２５）。Ｐ５３蛋白的表达与囊腺癌不同组织学分级和临床分期之间无相关性（Ｐ＞０．０５）。图像定量分析显示，囊腺癌Ｐ５３蛋白阳性细胞平均积分光密度值（３．６３±２．２０）显著高于交界瘤的值（１．６８±３．４９），（Ｐ＜０．００１）。ＤＮＡ含量图像分析显示，Ｐ５３蛋白阳性组，肿瘤细胞ＤＮＡ指数总体平均值（１．８８±０．２３）显著大于Ｐ５３蛋白阴性组的ＤＮＡ指数的总体平均值（１．３４±０．３３），（Ｐ＜０．００１）；≥５ｃ的肿瘤细胞百分率（３４．１％）显著高于阴性组（６．９％）（Ｐ＜０．００５）。结论Ｐ５３基因突变参与了卵巢粘液性肿瘤由良性向恶性转化的过程，Ｐ５３蛋白的表达可能作为该肿瘤恶性转化的标志，Ｐ５３蛋白表达与ＤＮＡ含量关系密切。

The relationship between the expression of P53 protein and the DNA content in the ovarian mucinous tumours

Objective To study the relationship between the expression of P53 protein in the ovarian mucinous tumours and the pathological histology types, grading, clinical phase and DNA content. Methods Expressions of P53 protein in 65 cases of ovarian mucinous tumours were observed by immunohistochemistry and DNA analysis. Results The expression rate of P53 protein in ovarian mucinous tumours cystadenomas was negative, in BMC 17.4% and in MCC 50.0%. The expression rate of MCC was significantly higher than that of benign MC (p<0.001) and BMC (p<0.025). The expression of P53 protein was not related to the histological grading and clinical phase of MCC (p>0.05). Image analysis showed that the positive cells of P53 protein in MCC (3.632.20) was significantly higher than that of BMC (1.683.49)(p<0.001). DNA analysis showed that in the P53 protein positive group, the population mean of those tumour cell DI s (DNA Index) (1.880.23) was significantly higher than that of the P53 protein negative group (1.340.33)(P<0 .001) and the percentage of those5c tumour cells (34.19%) is higher than that of the negative group (6.9%) (p<0.005). Conclusions The expression of P53 protein might be the mark of the transformation of ovarian mucinous tumours from the benign to malignant state. Very close relationship is found between the expression of P53 protein and DNA content.