| 鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响 |
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| 引用本文： | 张萍,袁丽杰,赵蕾,李百祥.鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响[J].癌变．畸变．突变,2009,21(6):418-421. |
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| 作者姓名： | 张萍 袁丽杰 赵蕾 李百祥 |
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| 作者单位： | 1. 哈尔滨医科大学大庆校区医学检验系,黑龙江,大庆163319
2. 哈尔滨医科大学公共卫生学院毒理学教研室,黑龙江哈尔滨,150081 |
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| 基金项目： | 国家自然科学基金项目(30170806) |
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| 摘 要： | 背景与目的：碱性鞘磷脂酶（alk-SMase）是鞘磷脂（sM）新陈代谢和发挥抑制结肠癌作用的关键酶。通过体外实验探讨sM的水解产物的神经酰胺（Cer）和鞘氨醇（Sph）对人结肠癌细胞HT-29中alk-SMase表达的影响。材料与方法：分别用12．5、25、50P-mol／L的CrCer和Sph处理HT-29细胞12、24、48h后，分别用Westernblot法和RT-PCR检测细胞中alk-SMase的蛋白水平和mRNA表达水平的变化。实验并设DMSO溶剂对照组。结果：C2-Cer和Sph作用HT-29细胞后，其alk-SMase蛋白及其mRNA的表达下调。结论：Cer和Soh对HT-29细胞中alk-SMase的表达具有负反馈调节作用。
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| 关 键 词： | 神经酰胺 鞘氨醇 碱性鞘磷脂酶 人结肠癌HT-29细胞 |
Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell |
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| ZHANG Ping,YUAN Li-jie,ZHAO Lei,LI Bai-xiang.Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell[J].Carcinogenesis,Teratogenesis and Mutagenesis,2009,21(6):418-421. |
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| Authors: | ZHANG Ping YUAN Li-jie ZHAO Lei LI Bai-xiang |
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| Institution: | ZHANG Ping1,YUAN Li-jie1,ZHAO Lei1,LI Bai-xiang2(1.Department of Biological Chemistry,Daqing Campus of Harbin Medical University,Daqing 163319,Heilongjiang,2.Department of Toxicology,Public Health College,Harbin Medical University,Harbin 150081,China) |
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| Abstract: | BACKGROUND AND AIM: Alkaline sphingomyelinase(alk-SMase) is the key enzyme of sphingomyelin(SM) metabolism, and an inhibitor of colon cancer. Through vitro experiments, we examined the alk-SMase expression in human colon cancer HT-29 cells, which produced the hydrolysis. products of SM, ceramide(Cer) and sphingosine(Sph). MATERIALS AND METHODS: After treatment with 12.5,25 and 50 μmol/L of C_2-Cer and Sph for 12,24 and 48 hours, we tested the alk-SMase mRNA and protein expressions in HT-29 cells. DMSO was the control. RESULTS: Compared with the control group, the alk-SMase expression of protein and mRNA level was reduced by C_2-Cer and Sph in HT-29 cells, With a clear dose-response relationship. CONCLUSION: Cer and Sph exerted negative feedback on alk-SMase. Cer and Sph might have induced apoptosis directly in colon cancer cells, rather than through hydrolysis of SM. |
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| Keywords: | ceramide sphingosine alkaline sphingomyelinase colon cancer HT-29 cells |
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