姜黄素抑制结肠癌SW480细胞的增殖及其机制

目的:研究姜黄素对人结肠癌SW480细胞增殖以及survivin、caspase-3和p-Akt表达的影响。方法:用不同浓度姜黄素(5～40μmol/L)作用SW480细胞24、48和72 h,MTT法检测姜黄素对SW480细胞生长的抑制作用,Western blotting法检测SW480细胞中survivin、caspase-3和p-Akt蛋白的表达。结果:姜黄素以剂量(5～40μmol/L)和时间(24～72 h)依赖的方式抑制SW480细胞的增殖(P<0.01),40μmol/L姜黄素作用72 h对SW480细胞生长的抑制率可达(75.86±3.93)%。姜黄素抑制SW480细胞中p-Akt、survivin蛋白的表达、促进caspase-3蛋白的表达,均呈时间和剂量依赖性;40μmol/L姜黄素作用SW480细胞48 h后p-Akt和survivin蛋白表达显著减少(0.204±0.025)vs(0.367±0.035),P<0.01;(0.208±0.014)vs(0.385±0.034),P<0.01],caspase-3蛋白表达显著增加(0.371±0.028)vs(0.127±0.023),P<0.01]。结论:姜黄素抑制SW480细胞增殖,其机制可能与抑制Akt磷酸化、下调survivin和上调caspase-3蛋白的表达有关。

Inhibitory effect of curcumin on proliferation of colon cancer SW480 cells and its mechanism

Objective: To study the effect of curcumin on proliferation and expression of survivin,caspase-3 and p-Akt in colon cancer SW480 cells.Methods: SW480 cells were treated with different concentrations of curcumin(5-40 μmol/L) for 24,48 and 72 h.The inhibitory effect of curcumin on SW480 cells was detected by MTT.The expressions of survivin,caspase-3 and p-Akt protein were analyzed by Western blotting.Results: Curcumin decreased the proliferation of SW480 cells in a dose-and time-dependent manner(P < 0.01),with an inhibitory rate of(75.86 ± 3.93) % after 40 μmol/L curcumin treatment for 72 h.Curcumin decreased the expressions of p-Akt and survivin,and increased the expression of caspase-3 protein in a dose-and time-dependent manner(P < 0.01).Treated with curcumin(40 μmol/L) for 48 h,the expressions of p-Akt and survivin protein were decreased significantly(0.204 ± 0.025]vs 0.367 ± 0.035],P < 0.01;0.208 ± 0.014]vs 0.385 ± 0.034],P < 0.01),and the expression of caspase-3 protein was increased significantly(0.371 ± 0.028] vs 0.127 ± 0.023 ],P < 0.01).Conclusion: Cureumin inhibits the proliferation of SW480 cells,which may be related to a decrease in phosphorylation of Akt,down-regulation of survivin protein expression and up-regulation of caspase-3 protein expression.