lncRNA 00707 通过miR-613 调控胃癌MGC-803、SGC-7901 细胞的恶性生物学行为

摘要] 目的:探讨长链非编码RNA 00707（lncRNA 00707）和微小RNA-613(miR-613)对胃癌细胞增殖和转移的调控作用及其相关机制。方法：收集2014 年1 月至2018 年6 月青海省人民医院肿瘤外科89 例原发性胃癌组织及癌旁组织标本以及胃癌MGC-803、SGC-790、BGC-823 细胞，采用qPCR实验检测胃癌组织和细胞系中lncRNA 00707、miR-613 的表达水平。分别建立lncRNA00707 低表达和过表达细胞模型，采用CCK-8 实验检测MGC-803、SGC-7901 细胞增殖能力，Transwell 法检测MGC-803、SGC-7901 细胞迁移和侵袭能力。用双荧光素酶基因报告实验验证lncRNA 00707 与miR-613 的靶向关系。结果：与癌旁组织相比，胃癌组织中lncRNA 00707 呈明显高表达(P<0.01)，lncRNA 00707 表达水平与WHO分期呈正相关(P<0.05)；与正常细胞系GES-1 相比，胃癌细胞系中lncRNA 00707 表达明显上调(P<0.05)。敲低lncRNA 00707 可明显抑制SGC-7901 细胞的增殖、侵袭和迁移能力(均P<0.05)；lncRNA 00707 过表达可明显提高MGC-803 细胞的增殖和迁移能力(P<0.05)。与阴性对照组相比，lncRNA00707 过表达显著降低了miR-613 荧光素酶报告载体的荧光素酶活性，而下调MGC-803 和SCG-7901 细胞中lncRNA 00707，miR-613 的表达显著增加（均P＜0.01）。结论：lncRNA 00707 在胃癌组织和细胞系中发挥促癌效应，其可以通过抑制miR-613 而促进胃癌MGC-803 和SCG-7901细胞的增殖和转移。

lncRNA 00707 regulates malignant biological behavior of gastric cancer MGC-803 and SGC-7901 cells via miR-613

Abstract] Objective: To investigate the role of long chain non-coding RNA00707 (lncRNA 00707) and micro RNA-613 (miR-613) in regulating the proliferation and metastasis of gastric cancer cells and its underlying mechanisms. Methods: Eighty-nine pairs of primary gastric cancer tissues and corresponding prar-cancerous tissues were collected from the Department of Surgical Oncology, Qinghai Provincial People''s Hospital during January 2014 and June 2018 for this study. The expressions of lncRNA 00707 and miR-613 in gastric cancer tissues and cells were detected by qPCR. The lncRNA 00707 low expression and over-expression models of MGC-803 and SGC-7901 cells were established; The proliferation of gastric cancer cells was monitored by CCK-8 assay, and Transwell assay was performed to determine the migration and invasion of gastric cancer cells. Dual luciferase gene reporter assay was adopted to validate the relationship between lncRNA 00707 and miR-613. Results: Compared with para-cancerous tissues and normal cell line GES-1, the expression of lncRNA 00707 was significantly up-regulated in cancer tissues and cell lines, and the expression of lncRNA 00707 was positively correlated with WHO stage (all P<0.05). Down-regulation of lncRNA 00707 significantly inhibited the proliferation and migration of SGC-7901 cells, while overexpression of lncRNA00707 exerted the opposite effect (all P<0.05). Compared with negative control group, lncRNA00707 over-expression significantly reduced the luciferase activity of miR-613; in the contrary, the luciferase activity of miR-163 was significantly increased in MGC-803 and SGC-7901 cells with lncRNA 00707 knockdown (all P<0.01). Conclusion: lncRNA 00707 facilitates the proliferation,migration and invasion of gastric cancer cells by inhibiting the function of miR-613, which exerts a protumorigenic effect in gastric cancer.