肿瘤坏死因子相关凋亡诱导配体对白血病NB4和K562细胞的作用及与其受体表达的关系

 【摘要】　目的　探讨肿瘤坏死因子相关凋亡诱导配体 （TRAIL）对白血病NB4和K562细胞的作用及与其受体表达的关系。方法　以Jurkat细胞株为阳性对照，采用不同浓度的TRAIL分别作用于NB4和K562细胞，观察细胞形态；采用四甲基偶氮唑蓝（MTT）比色法检测细胞生长情况；用流式细胞术检测细胞表面TRAIL受体的表达情况。结果　TRAIL作用导致NB4细胞株生存率显著下降，但弱于Jurkat细胞株，其变化具有TRAIL作用时间和浓度依赖性；对K562细胞株生存率的影响不明显。NB4细胞表面死亡受体4（DR4）和DR5表达水平较高，同时诱骗受体1（DcR1）表达较高；K562细胞DR5表达水平较高，而DR4及DcR1微量表达；Jurkat细胞表面仅DR5低水平表达；DcR2在三株细胞表面均无表达。结论　NB4细胞对TRAIL中度敏感，K562细胞对TRAIL耐受；NB4细胞敏感性较低可能与DcR1表达有关，K562细胞耐受性与其表面TRAIL受体表达无关。

Effect of TRAIL on NB4 and K562 cell lines and its relationship with TRAIL receptors expression

Objective To investigate the effect of TRAIL on NB4 and K562 cell lines, and its relationship with TRAIL releptors. Methods Jurkat cells were used as positive control, NB4 and K562 cells were treated with different concentrations Of TRAIL. Cell morphologic changes were monitored. The cell proliferation was evaluated by MTT assay. The expression of TRAIL receptor were determined by flow cytometry. Results MTT assay showed that TRAIL inhibited the growth of NB4 and Jurkat cells in vitro in a dose-and time-dependent manner, but the effect of TRAIL on Jurkat cells was stronger than that on NB4 cells. However, the growth of K562 was not inhibited. Flow eytometry analysis revealed that DR4,DR5 and DeR1 were expressed higher in NB4 and K562 cells, but the levels of DR4 and DcR1 were very low in K562 cells. DR5 was expressed in Jurakat cells with low level. No DcR2 was detected on the surface of all the three cell lines. Conclusion NB4 cell line is moderately sensitive to TRAIL, and K562 cell line is resistant to TRAIL. The sensitivity of NB4 cells to TRAIL may be associated with the expression of DcR1, but the sensitivity of K562 cells have nothing tO do with the expression of TRAIL receptors.