| 肺癌细胞中RASSF4的高表达抑制肺癌细胞的增殖和侵袭能力 |
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| 引用本文： | 刘洋,韩勇,王恩华.肺癌细胞中RASSF4的高表达抑制肺癌细胞的增殖和侵袭能力[J].现代肿瘤医学,2016,0(14):2190-2194. |
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| 作者姓名： | 刘洋 韩勇 王恩华 |
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| 作者单位： | 中国医科大学基础医学院病理学教研室，辽宁 沈阳 110001 |
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| 基金项目： | 国家自然科学基金(编号30900562)；教育部高等学校博士学科点专项科研基金(编号20092104120022)；辽宁省教育厅科学研究一般项目(编号L2015583) |
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| 摘 要： | 目的:探讨RASSF4在肺癌中的增殖和侵袭能力。方法:向肺癌细胞系H460和A549中导入RASSF4的cDNA质粒后，通过MTT、克隆形成实验、基质胶侵袭实验、流式细胞术等方法检测肺癌细胞的生长和侵袭能力。结果:RASSF4能够通过下调MMP2、MMP9和cyclinD1的表达，抑制肺癌细胞的侵袭、增殖及克隆形成能力。结论:RASSF4在肺癌细胞中通过抑制细胞生长及侵袭能力发挥重要的肿瘤抑制功能。
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| 关 键 词： | RASSF4 肺癌 增殖 侵袭 |
Decreased expression of Pygopus 2 suppresses cell growth in lung cancer cell lines |
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| Liu Yang,Han Yong,Wang Enhua.Decreased expression of Pygopus 2 suppresses cell growth in lung cancer cell lines[J].Journal of Modern Oncology,2016,0(14):2190-2194. |
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| Authors: | Liu Yang Han Yong Wang Enhua |
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| Institution: | Department of Pathology,The Basic Medical Sciences of China Medical University,Liaoning Shenyang 110001,China. |
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| Abstract: | Objective:Despite the results showed that RASSF4 play the tumor suppressor role in tumor cells,no studies investigating its role in human non - small cell lung cancer( NSCLC). Methods:In the present study, RASSF4 plasmid transfection was performed in H460 and A549 cell lines. The cell growth was observed using a3 -(4,5 - dimethylthiazol - 2 - yl)- 2,5 - diphenyltetrazolium bromide(MTT)assay,flow cytometry analysis and colo-ny formation. The invasive ability of cell was observed by Matrigel invasion assay. Results:RASSF4 overexpression in-hibited proliferation,colony formation,and invading ability with downregulation of cyclin D1,MMP2 and MMP9. Con-clusion:RASSF4 was a tumor suppressor in NSCLC. |
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| Keywords: | RASSF4 lung cancer proliferation invasion |
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