GDDR 对 5 - FU 诱导下胃癌细胞凋亡的影响

目的：通过5-Fu诱导人胃癌细胞系SGC-7901凋亡，研究GDDR在胃癌细胞凋亡中的作用。方法：利用已构建成功的SGC-7901-GDDR和SGC-7901-pcDNA3．1稳定转染细胞系，在5-Fu诱导下促进其凋亡发生，流式细胞术和Westernblot检测以上两种细胞系中凋亡发生率和Caspase-3表达及活化程度。结果：未加药组中SGC-7901-GDDR和SGC-7901-pcDNA3．1凋亡发生率无统计学意义，且两者均未观察到活化型Caspase-3表达。加药组中SGC-7901-GDDR凋亡发生率显著高于SGC-7901-pcDNA3．1，且活化型Caspase-3表达前者显著高于后者，差异具有统计学意义（P〈0．05）。结论：GDDR可通过上调活化型Caspase-3的表达促进SGC-7901细胞凋亡，提示凋亡可能也是GDDR抑癌途径之-，并因此可能在胃肠道肿瘤治疗中发挥作用。

GDDR enhances Caspase -3 activation through 5 - FU induced apoptosis in SGC -7901

Objective：To investigate the role of GDDR in the apoptosis of SGC -7901 cell line. Methods：Previ- ously,we have established the SGC- 7901 -GDDR which expresses GDDR stably and SGC -7901 -pcDNA3.1 as the control cell line. Apoptosis was evaluated using flow cytometry after the two cell lines treated with 5 - FU for 48 hours. The expression of cleaved Caspase - 3 was analyzed by Western blot. Results： After treated with 5 - FU for 48 hours,the apoptosis of SGC -7901 - GDDR was extremely higher compared with SGC -7901 - pcDNA3. 1. This change was reflected by a remarkable increase in the expression levels of cleaved Caspase - 3 （ P 〈 0.05 ）. While in the unteated group, there was no obvious differences observed. Conclusion： The increased expression of cleaved Caspase -3 upregulated by GDDR may play an important role in the apoptosis of gastric cancer,which indicated that GDDR might be an interesting molecular to apply in cancer intervention.