MnTBAP对大鼠急性放射性肺损伤保护作用的实验研究

目的:观察锰四(4-苯甲酸)卟啉[MnTBAP]对大鼠急性放射性肺损伤的保护作用。方法:200～230g SD雄性大鼠分为正常对照组、照射损伤组和MnTBAP治疗组。用6 MV X射线、单次照射全肺28Gy,建立大鼠肺损伤模型。损伤组腹腔注射生理盐水(1.5mL/kg),MnTBAP组腹腔注射MnTBAP(10mg/kg)。经1、4、8和12周,采用ELISA法检测血清转化生长因子β1(TGF-β1)水平;肺组织作病理检查和生化检测超氧化物歧化酶(SOD)、脂质过氧化产物丙二醛(MDA)、羟脯氨酸(Hyp)、抗超氧阴离子自由基(ASAR)。结果:病理检查显示,MnTBAP组肺组织损伤程度较损伤组明显减轻;生化检测结果显示,经1～12周,与照射损伤组相比较,MnTBAP治疗后SOD含量显著增加(P值均<0.001),ASAR含量显著增加(P值均<0.001),MDA含量显著减少(1周,P<0.001;4～12周,P值均<0.05),TGF-β1含量显著减少(P值均<0.001),Hyp含量1～4周没有变化,8～12周明显降低(8周,P<0.05;12周,P<0.01)。结论:MnTBAP具有抗氧化清除自由基功能,对放射性肺损伤具有保护作用,可能是潜在的放射性损伤保护剂。

Protection effect of MnTBAP on acute radiation-induced lung injury in rats

OBJECTIVE:To investigate the protective effect of MnTBAP on radiation-induced lung injury in the rats model.METHODS: Male SD rats were divided into three groups:control group,radiation injured group,and MnTBAP treated group.lung injury models were made by putting the rats under the exposure of 6 MV X-ray at a dose of 28 Gy thoracically.Then the normal saline was injured intraperitoneally immediately of those rats in the injured group,while,all other things equal,MnTBAP(10 mg/kg) was injected to the treated group.After 1,4,8 and 12 weeks,using biochemical methods to test the superoxide dismutase(SOD),malondialdehyde(MDA),anti-superoxide anion radicals(ASAR) activity,hydroxyproline(Hyp) and the serum of transforming growth factor-β1(TGF-β1) level.RESULTS: When comparing these two groups,the pathological test shows that the injury level of MnTBAP group was much slighter than that of injured group.Meanwhile,biochemical test result shows that the content of SOD increased notably after 1 to 12 weeks time(all P<0.001),in addition the ASAR incresed significantly too(all P<0.001).On the contrary,the amount of MDA significantly decreased(1 week,P<0.001;4-12 weeks,all P<0.05),the amount of TGF-β1 significantly decreased(all P<0.001).Moreover,the Hyp,stayed at the same level for the first 4 weeks but reduced significantly during 8-12 weeks(8 weeks,P<0.05;12 weeks,P<0.01).CONCLUSIONS: MnTBAP has the function of-antioxidant and the function of eliminating the free radical.It also has the protecting function to radiation-induced lung injury.In this case,it may be a potential radio-protector.