| RNA干涉在肿瘤研究中的应用进展 |
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| 引用本文： | 亓同钢,汪运山.RNA干涉在肿瘤研究中的应用进展[J].中华肿瘤防治杂志,2003,10(12):1333-1335. |
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| 作者姓名： | 亓同钢 汪运山 |
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| 作者单位： | 1. 山东大学第二医院临床分子生物学实验室,山东,济南,250033
2. 山东省移植与组织工程技术研究中心,山东,济南,250013 |
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| 摘 要： | RNA干涉 (RNAinterference ,RNAi)是生物体内的一种通过双链RNA(double strandedRNA ,dsRNA)来抵抗病毒入侵和抑制转座子活动的自然机制 ,近来研究发现 ,2 1～ 2 5nt大小的小干涉RNA(smallinterferingRNA ,siRNA)可在哺乳动物细胞中介导高度序列特异性的基因沉默 ,并且具有高效性和序列特异性 ,已经成为功能基因组研究的有力工具 ,并且有可能在某些疾病如肿瘤等的基因治疗中发挥重要作用
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| 关 键 词： | RNA干涉 肿瘤 基因沉默 siRNA 综述文献 |
| 文章编号： | 1009-4571(2003)12-1333-03 |
| 修稿时间： | 2003年7月10日 |
Advances in Research of RNA Interference and Its Use in Cancer |
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| QI Tong-gang,WANG Yun-shan.Molecular Biology Laboratory,the Second Hospital,Shandong University,Jinan ,China.Advances in Research of RNA Interference and Its Use in Cancer[J].Chinese Journal of Cancer Prevention and Treatment,2003,10(12):1333-1335. |
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| Authors: | QI Tong-gang WANG Yun-shanMolecular Biology Laboratory the Second Hospital Shandong University Jinan China |
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| Institution: | QI Tong-gang,WANG Yun-shan.Molecular Biology Laboratory,the Second Hospital,Shandong University,Jinan 250033,China |
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| Abstract: | RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell,causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences,including endogenous mRNAs.Several rapidly developing RNA interference (RNAi) methodologies hold the promise to selectively silence gene expression in mammals.As such,RNAi technology is currently being evaluated not only as an extremely powerful technology for functional genomic analyses,but also as a potentially useful method to develop highly specific dsRNA based gene-silencing therapeutics.Here,this paper reviews the progress in the research of RNA interference and its use in cancer. |
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| Keywords: | RNAi neoplasms gene silence siRNA review literature |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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