| 胰腺癌组织转录因子GATA-6表达及其意义的探讨 |
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| 引用本文： | 陈文博,张世能,庄燕妍,庄晓虹,黄凤婷,唐健.胰腺癌组织转录因子GATA-6表达及其意义的探讨[J].中华肿瘤防治杂志,2011,18(14):1101-1104. |
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| 作者姓名： | 陈文博 张世能 庄燕妍 庄晓虹 黄凤婷 唐健 |
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| 作者单位： | 中山大学孙逸仙纪念医院消化内科,广东广州,510120 |
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| 基金项目： | 广东省自然科学基金(8151008901000139) |
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| 摘 要： | 目的:探讨胰腺癌组织GATA-6的表达及其对胰腺癌发生、发展的可能作用及临床意义。方法:采用免疫组化检测33例胰腺癌、15例慢性胰腺炎和6例正常胰腺组织GATA-6蛋白的表达;免疫细胞荧光法检测胰腺癌细胞株Panc-1、BxPC-3和SW1990及正常胰腺导管上皮细胞H6C7的GATA-6蛋白表达情况;Real-time RT-PCR技术检测上述细胞株的GATA-6 mRNA表达;并分析GATA-6与胰腺癌临床病理参数的关系。结果:GATA-6蛋白在胞核中表达,胰腺癌组织GATA-6的阳性表达率(88.9%)显著高于慢性胰腺炎(40.0%)和正常胰腺组织(16.7%),P<0.01,但与性别、年龄、分化程度和临床分期均无显著性相关;3种胰腺癌细胞株均有GATA-6蛋白表达,而正常胰腺导管上皮细胞无表达;3种胰腺癌细胞株GATA-6 mRNA表达也明显高于正常胰腺导管上皮细胞,P<0.05。结论:GATA-6在胰腺癌组织及细胞中呈高表达,提示GATA-6在胰腺癌的发生、发展中可能起一定作用。
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| 关 键 词： | 胰腺肿瘤 GATA-6 免疫组化 免疫荧光化学 |
Expression of GATA-6 in pancreatic carcinoma tissues and its significance |
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| CHEN Wen-bo,ZHANG Shi-neng,ZHUANG Yan-yan,ZHUANG Xiao-hong,HUANG Feng-ting,TANG Jian.Expression of GATA-6 in pancreatic carcinoma tissues and its significance[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(14):1101-1104. |
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| Authors: | CHEN Wen-bo ZHANG Shi-neng ZHUANG Yan-yan ZHUANG Xiao-hong HUANG Feng-ting TANG Jian |
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| Institution: | CHEN Wen-bo,ZHANG Shi-neng,ZHUANG Yan-yan,ZHUANG Xiao-hong,HUANG Feng-ting,TANG Jian Department of Gastroenterology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,P.R.China |
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| Abstract: | OBJECTIVE: To investigate the expression and it's clinical significance in the carcinogenesis and development of pancreatic adenocarcinoma.METHODS: HRP immunohistochemistry was used to detect the expression level of GATA-6 protein in patients with pancreatic carcinoma(33 cases),chronic pancreatitis(15 cases) and normal pancreas tissues(6 cases).Cell immunofluorescence and Real-time RT-PCR were used to detect the expression level of GATA-6 protein and mRNA in three pancreatic cancer cell lines and one human ... |
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| Keywords: | pancreatic neoplasm GATA-6 immunohistochemistry immunofluorescence |
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