肝细胞癌p53和VEGF的表达及其与肿瘤血管形成的关系

目的 :研究p5 3和血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)的表达和肿瘤微血管密度 (microvesseldensity ,MVD)测定在人肝细胞癌 (HCC)中的意义。方法 :采用免疫组织化学方法 ,检测 5 0例HCC患者手术切除标本p5 3和VEGF的表达 ,并用抗CD3 4 抗体标记癌组织血管内皮细胞 ,计算MVD。结果 :p5 3、VEGF总阳性表达率分别为 5 4 0 % (2 7 5 0 )和 76 0 % (38 5 0 ) ,p5 3、VEGF的表达和MVD均与HCC组织病理分级呈正相关 ,P <0 0 5。p5 3和VEGF的阳性表达符合率为 74 % (37 5 0 ) ,两者的表达呈相关性 ,P <0 0 5。p5 3阳性和VEGF阳性的癌组织MVD分别为 178± 6 2和 175±5 9 ,而相应的阴性组分别为 12 5± 5 1和 131± 6 1,两者差异有显著意义 ,P <0 0 5。p5 3、VEGF表达均为阳性者 ,MVD为 176± 6 3;p5 3、VEGF的表达均为阴性者 ,MVD为 12 3± 5 2 ;两者差异有显著意义 ,P <0 0 5。结论 :1)p5 3、VEGF的表达以及MVD的测定可作为判断HCC恶性潜能的重要生物学指标 ;2 )p5 3、VEGF的表达对肿瘤血管形成可能起重要作用 ,联合检测p5 3、VEGF的表达对了解肿瘤血管形成的机制有一定意义

P53 and Vascular Endothelial Growth Factor Expression in Hepatocellula Carcinoma and Their Relation to Angiogenesis

Objective To investigate the relation of expression of p53 gene and vascular endothelial growth factor (VEGF) to angiogenesis of hepatocellula carcinoma (HCC).Methods The surgical specimens from 50 cases of HCC were stained immunohistochemically for the experssion of p53 and MVD. MVD was calculated by labeling the endothelial cells of the blood vessels within the tumor.Results p53,VEGF expressions were closely correlated with histopathogical grade of HCC.Positive rates of p53 protein and VEGF expressions were 54.0% (27/50) and 76%(38/50) respectively.The conformation rate of p53 and VEGF was 74%(37/50).MVD was significantly higher in the p53-positive or VEGF-positive tumors than that in the negative ones,P<0.05.Conclusions p53 protein,VEGF and MVD can be considered as biological indicators of malignant potential in HCC patients.p53 and VEGF expressions,both contributing to the tumor neovascularization, may be helpful to the understanding of tumoral angiogenetic mechanism in the future.