IS201对GL15胶质瘤细胞FAK、ERK1/2蛋白表达及磷酸化的影响

背景与目的：之前已有研究证明,整合素参与调节细胞多种生物学效应;黏着斑激酶（FAK）、细胞外调节蛋白激酶（ERK1/2）蛋白表达及磷酸化的水平与胶质瘤细胞恶性程度及其诊断、预后密切相关。本研究通过检测整合素αvβ3拮抗剂IS201对人GL15胶质瘤细胞FAK、ERK1/2蛋白表达及其磷酸化的影响,探讨其意义。方法：用不同浓度的整合素αvβ3拮抗剂IS201处理GL15细胞,用Western印迹法检测细胞的FAK、ERK1/2表达量以及FAK、ERK1/2的磷酸化程度。结果：各实验组不同浓度的IS201均能明显降低FAK、ERK1/2的表达,对FAK、ERK1/2的磷酸化有明显的抑制作用。各实验组差异均具有统计学意义（P〈0.05）。结论：IS201对人GL15胶质瘤细胞FAK、ERK1/2蛋白表达量及其磷酸化均起负性调节作用,对胶质瘤的细胞增殖和侵袭性生长等恶性生物学行为起抑制作用。

Effect of IS201 on FAK, ERK1/2 Protein Expression and Their Phosphorylation in GL15 Glioma Cells

BACKGROUND OBJECTIVE： Integrin is a promising target for malignant gliomas. In this article, we evaluated influence of the integrin αvβ3 antagonist IS201 on the expression and phosphorylation of FAK, ERK1/2 in glioma cell line GL15 . METHODS： Western-blotting was used to detect the effect of IS201 at different concentration on the expression and phosphorylation of FAK, ERK1/2 in glioma cell line GL15,. RESULTS： IS201 significantly reduced the expression and inhibited the phosphorylation of FAK, ERK1/2 at different concentration. Each experimental group showed statistical difference （P 0.05）. CONCLUSION： IS201 negatively regulates the expression and phosphorylation of FAK and ERK1/2 in human GL15 glioma cells and potentially inhibit proliferation and invasive growth of malignant glioma.