人脑胶质瘤组织中fibulin-5的表达及意义

目的探讨fibulin-5在人脑胶质瘤组织中的表达及其临床意义。方法通过免疫组织化学方法结合图像分析，对正常脑组织和胶质瘤组织进行fibulin-5蛋白表达差异分析。结果fibulin-5主要分布于细胞之间，实质细胞及血管内皮细胞胞质亦可检测到其表达。fibulin-5在7例正常脑组织均检测到表达（100%）；在高级别胶质瘤组中，19例标本有4例检测到表达(22%)；在低级别胶质瘤组中，21例标本有13例检测到表达(62%)。正常脑组织标本的fibulin-5蛋白表达阳性率以及平均IRS值（immuno-reactive score免疫阳性反应积分）与低级别胶质瘤组相比差异无统计学意义（P>0.05）；高级别胶质瘤组的fibulin-5蛋白表达阳性率以及平均IRS值均低于正常脑组织和低级别胶质瘤组的表达，差异有统计学意义（P<0.05）。在胶质瘤组织中，fibulin-5蛋白的表达在不同性别及年龄上差异无统计学意义（P>0.05）。结论fibulin-5在正常脑组织中表达，其在胶质瘤组织中表达的变化可能与胶质瘤的进展和血管生成存在联系。

Expression and Significance of fibulin-5 in Human Glioma Tissues

Objective To investigate the expression and clinical significance of fibulin-5 in human glioma tissues. Methods The different expression of fibulin-5 in normal brain tissue and glioma are analyzed by immunohistochemical stain combined with image analysis. Results Fibulin-5 is mainly distributed in cells, and its expression in parenchymal cells and vascular endothelial cells also can be detected. Fibulin-5 expresses in all seven cases of normal brain tissue (100%), and in 4 of 19 in the high-grade glioma cases (22%), and in 13 of 21 in low grade glioma group cases (62%).The positive expression rate of the fibulin-5 protein and the average immuno-reactive score points have no statistical difference between normal brain tissue samples and low-grade gliomas (P>0.05); The positive expression rate of the fibulin-5 protein and the average immuno-reactive score points in high grade glioma are lower than normal brain tissue and low grade glioma, it has statistical difference (P<0.05). In glioma tissues, the expression of fibulin-5 protein in different sex and age has no statistical difference (P>0.05). Conclusion Fibulin-5 could is expressed in the normal brain tissue. The expression of fibulin-5 may correlate with the process and the angiogenesis of gliomas.