| 环磷酰胺联合卡介苗对Lewis肺癌小鼠CD4~+CD25~+Treg细胞及效应细胞功能的影响 |
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| 引用本文： | 李欣,崔永生,刘白楠,李一.环磷酰胺联合卡介苗对Lewis肺癌小鼠CD4~+CD25~+Treg细胞及效应细胞功能的影响[J].肿瘤防治研究,2008,35(10):694-696. |
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| 作者姓名： | 李欣 崔永生 刘白楠 李一 |
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| 作者单位： | 1. 吉林大学基础医学院免疫学教研室, 长春,130021
2. 130021 长春,吉林大学基础医学院免疫学教研室;吉林大学一医院胸外科 |
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| 摘 要： | 目的探讨环磷酰胺(CTX)联合卡介苗(BCG)治疗对Lewis肺癌小鼠CD4+CD25+Treg细胞和效应细胞功能的影响及CD4+CD25+Treg细胞与肿瘤的相关性。方法将传代培养的Lewis肺癌细胞接种于C57BL/6小鼠右腋皮下,建立Lewis肺癌模型。采用CTX联合BCG治疗,观察各组动物的肿瘤体积、脾脏CD4+CD25+Treg细胞数量和Foxp3 mRNA表达水平、脾脏T淋巴细胞增殖和杀伤功能。结果CTX联合BCG治疗组肿瘤生长较肿瘤组缓慢;联合治疗组小鼠脾脏CD4+CD25+Treg细胞数量明显低于肿瘤组(P<0.05);联合治疗组小鼠脾脏Foxp3 mRNA表达水平明显低于肿瘤组(P<0.05);联合治疗组小鼠脾脏T淋巴细胞增殖功能明显高于肿瘤组(P<0.05);联合治疗组小鼠脾脏CTLs细胞的杀伤活性略高于肿瘤组(P>0.05)。结论CTX联合BCG治疗可明显降低CD4+CD25+Treg细胞数量和Foxp3 mRNA表达水平,并增强机体抗肿瘤免疫应答,使肿瘤生长延缓。
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| 关 键 词： | CTX Treg Foxp3 肿瘤免疫 |
| 收稿时间： | 2007-10-15 |
| 修稿时间： | 2008-1-21 |
Function of CD4~+CD25~+Treg and Effecter Cells of Mice with Lewis Lung Cancer Were Influenced by CTX and BCG Therapeutic Alliance |
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| LI Xin,CUI Yong-sheng,LIU Bai-nan,LI Yi.Function of CD4~+CD25~+Treg and Effecter Cells of Mice with Lewis Lung Cancer Were Influenced by CTX and BCG Therapeutic Alliance[J].Cancer Research on Prevention and Treatment,2008,35(10):694-696. |
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| Authors: | LI Xin CUI Yong-sheng LIU Bai-nan LI Yi |
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| Institution: | LI Xin1,CUI Yong-sheng1,2,LIU Bai-nan1,LI Yi11.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China,2.Department of Thoracic Surgery,First Hospital |
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| Abstract: | Objective To study the function of CD4+CD25+Treg and effecter cells of mice with Lewis lung cancer were influenced by CTX and BCG therapeutic alliance,investigate the relationship of CD4+CD25+ Treg and the tumor,and provide experiment evidence for the tumor immunotherapy.MethodsThe models were established by injected CTX(25 mg/kg) and after 7 days injected subcutaneously to the right axilla of C57BL/6 mice with subculturing Lewis lung cancer cells and BCG(12.5 mg/kg).The dynamic changes of tumor volume were... |
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| Keywords: | CTX Treg Foxp3 |
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