TRAIL对原代卵巢癌细胞增殖、凋亡及核 因子κB的影响

 目的 观察肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptosis inducing- ligand； TRAIL）对原代卵巢癌细胞生长的抑制和诱导肿瘤细胞凋亡效应，以及细胞核因子κB(NF κＢ)激活和转运的影响。方法 来源于12例病人原代卵巢癌细胞，给予TRAIL 0、5、10、20、50、100μg/L分别作用24、48、72和96h，运用MTT比色法检测原代卵巢癌细胞生长、增殖情况；细胞免疫荧光法检测原代卵巢癌细胞凋亡和细胞内NF κB激活－转运情况。结果 TRAIL对原代卵巢癌细胞的生长抑制呈剂量依赖性，浓度大于20μg/L时抑制率增辐减慢；浓度相同时对癌细胞的抑制呈时间依赖性，72h后抑制率上升减缓；肿瘤细胞凋亡率随TRAIL作用时间延长而增加，5μg/L 24h、96h凋亡率分别是（28±1.18）%、（55±4.12）%；肿瘤细胞凋亡率也呈剂量依赖性；TRAIL作用后原代卵巢癌细胞质和胞核中NF κB表达增加。结论 TRAIL抑制原代卵巢癌细胞的增殖和生长，并诱导癌细胞的凋亡；激活癌细胞质中NF κB向细胞核内转运。

Effects of TRAIL on Proliferation,Apoptosis and NF-κB of Primary Human Ovarian Cancer Cell

Objective To investigate the regulatory effects of TRAIL on proliferation and apoptosis of primary human ovarian cancer cell in vitro，and the activation and transportation of NF-κＢ.Methods Primary human ovarian cancer cells were obtained from twelve patients.Each patients cell was divided to six groups，and treated with TRAIL (5～100 μg/L) for 24～96 h respectively.The ratio of growth inhibition was measured with MTT in primary human ovarian cancer cells.The ratio of apoptosis and the transportation and activation of NF κＢ were measured by immunofluorescence in primary human ovarian cancer cells.Results The ratio of growth inhibition increased in dose and time dependent manner when the concentration was 5，10 or 20 μg/L at 24,48,72h.The ratio of apoptosis increased along with the time lasting.It was (28±1.18)% with concentration of 100μg/L at 24h，and (55±4.12)% at 96h.The level of NF-κＢ in nucleus and cytoplasm was highly expressed.Conclusion TRAIL inhibits proliferation and growth of primary human ovarian cancer cell，and induces apoptosis.It enhances conveying the NF κＢ from cytolymph to nucleolus.