人NHE1 反义基因转染对SGC-7901 胃癌细胞增殖和凋亡的影响

 目的 探讨人NHE1反义基因转染对SGC-7901胃癌细胞株增殖和凋亡的影响，探索胃癌治疗的新方法。方法 采用脂质体法将构建好的人NHE1基因反义真核表达栽体转染至SGC-7901胃癌细胞中，比较观察细胞转染前后生长曲线、双层软琼脂克隆形成能力、裸鼠成瘤能力以及细胞周期和细胞凋亡率的变化。结果 NHE1反义基因能使SGC-7901胃癌细胞生长速度减慢，双层软琼脂集落形成能力、体外生长增殖能力降低，细胞凋亡率增加，裸鼠体内成瘤能力显著降低。结论 反义NHE1基因能抑制SGC-7901胃癌细胞增殖，诱导细胞凋亡，具有良好的胃癌治疗效果，提示NHE1基因可成为胃癌治疗的新靶点，具有一定的临床应用前景。

Effect of Antisense Gene to Human NHE1 on the Proliferation and Apoptosis of SGC-7901 after Transfection

Objective To study the effects of Antisense gene to human NHE1 on the proliferation and apoptosis of SGC-7901 after transefection and its potential role in the genetherapy for gastric cancer. Methods NHE1 antisense gene eukaryotic expression vector constructed was transfected into SC, C-7901 with liposome DOTAP. We examined the changes of the proliferation index, the clone formation capacity in two layer soft agar, growth curve ,apoptotic rates and nude mouse formation carcinoma capacity of the cells transfected antisense gene. Results Cells transfected NHE1 antisense gene compared with parent cells,Proliferation index decreased, the clone formation capacity in two layer soft agar play down, apoptotic rates increased, growth curve slowers. Format carcinoma capacity in nude mouse disappear. Conclusion Our study demonstrated that NHE1 antisense gene can restrain gastric carcinoma proliferation and induce carcinoma cell apoptosis which implied that may be a new target gene for antisense gene therapy of gastric cancer.