环状RNA ciRS-7在三阴性乳腺癌中的表达及其对细胞侵袭和迁移的影响

 目的 探讨环状RNA ciRS-7在三阴性乳腺癌（TNBC）中的表达及其对细胞侵袭和迁移的影响。方法 通过qRT-PCR法检测乳腺癌组织和细胞中ciRS-7的表达水平，分析其与乳腺癌患者临床病理指标之间的关系。用划痕实验和Transwell实验检测沉默ciRS-7后MDA-MB-231和BT-549细胞的迁移和侵袭能力的变化。利用动物实验在体内进行验证。结果 TNBC组织中ciRS-7的相对表达量为（6.52±0.38），显著高于Luminal型（1.56±0.17）（P<0.001）和HER2过表达型乳腺癌组织（2.27±0.66）（P<0.001）以及癌旁正常组织（0.83±0.09）（P<0.001）。ciRS-7表达与分子分型、肿瘤浸润和淋巴结转移明显相关（均P<0.05）；而与患者年龄、肿瘤直径和病理分级无关（均P>0.05）。沉默ciRS-7后，MDA-MB-231和BT-549细胞的迁移和侵袭明显减弱（均P<0.05）。ciRS-7敲低组肺转移瘤数明显减少（P<0.05）。结论 ciRS-7在TNBC中高表达，并可能增强TNBC细胞的侵袭和迁移。

Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells

Objective To investigate the expression of circular RNA ciRS-7 in triple-negative breast cancer (TNBC) and its effect on invasion and migration. Methods The expression levels of ciRS-7 in TNBC tissues and cells were detected by qRT-PCR, and its relationship with clinicopathological parameters of breast cancer patients was further analyzed. Scratch and Transwell assays were used to detect the migration and invasion of MDA-MB-231 and BT-549 cells after silencing ciRS-7. Animal experiment was performed to verify the above findings in vivo. Results The relative expression of ciRS-7 in TNBC tissues was (6.52±0.38), significantly higher than Luminal type (1.56±0.17) (P<0.001), HER2 overexpressing breast cancer tissues (2.27±0.66) (P<0.001) and normal adjacent tissues (0.83 ± 0.09) (P<0.001). Clinical data analysis showed that ciRS-7 expression was significantly associated with molecular typing, tumor invasion and lymph node metastasis (all P<0.05), not associated with patients’ age, tumor diameter or pathological grade (all P>0.05). After silencing ciRS-7, the migration and invasion of MDA-MB-231 and BT-549 cells were significantly reduced (all P<0.05). The number of lung metastasis clones was significantly reduced in ciRS-7 knockdown group (P<0.05). Conclusion ciRS-7 is highly expressed in TNBC and may enhance the invasion and migration of TNBC cells.