CHRNA3基因多态性与肺癌易感相关性分析

目的：探讨尼古丁乙酰胆碱受体基因CHRNA3多态性与肺癌易感性的关系。方法：采用以医院患者为基础的病例一对照研究方法，对2008-01-01-2011-06-30在江苏省肿瘤医院就诊原发性肺癌患者600例（病例组）和健康体检人员600名（正常对照组），以TaqMan-MGB探针技术检测CHRNA3上的SNPs位点rs3743073（T〉G）基因型，比较组间基因型分布频率的差异。结果：肺癌患者CHRNA3rs3743073（T〉G）的TT、TG和GG基因型频率分别为20．7％、43．0％和36．3％，正常对照人群分别为31．0％、48．5％和20．5％，差异均有统计学意义，P〈0．05。肺癌患者CHRNA3rs3743073（T〉G）的A、G等位基因频率分别为42．2％和57．8％，正常对照人群分别为53．3％和44．8％，差异均有统计学意义，P〈0．05。与TT基因型患者相比，TG和GG基因型患者发生肺癌的风险分别增加1．132倍（95％CI：1．004～1．277，P=0．048）和1．663倍（95％CI：1．406～1．968，P=0．001）；rs3743073G（TG和GG）患者发生肺癌的风险增加1．290倍（95％CI：1．150～1．447，P=0．001）。年龄〉60岁、男性、吸烟的患者中，rs3743073G变异基因型的患者发生肺癌的危险性显著增加，P=0．05。结论：CHRNA3基因rs3743073G变异基因型发生肺癌的危险明显增加，尤其在年龄〉60岁、男性和吸烟患者中更为显著。

Association between CHRNA3 gene polymorphisms and lung cancer risk

OBJECTIVE：To explore the association between CHRNA3 polymorphisms of nicotinic acetyleholine re ceptors gene and lung cancer risk via a hospital-based, case-control study. METHODS： Single nucleotide polymorphisms （SNPs） in CHRNA3 rs3743073 （T〉G） were determined by TaqMan-MGB probe technique in 600 lung cancer cases and 600 normal control people. The differences of genotype and allele frequency were compared between groups,and the asso ciation between its genotypes and lung cancer risk was studied. RESULTS： The genotype and allele frequency of rs3743073 （T〉G） were 20.7%,43.0%,36.3% and 42.2%,57.8% in lung cancer cases and 31.0%,48.5%,20.5% and 53.3%, 44.8 % in normal control people respectively, there were significantly difference in genotype and allele frequency between 2 groups （P〈0.05）. Compared patients with the TT genotypes,the risk of lung cancer in patients with TG and GG geno types increased significantly （ OR = 1. 132,95 % CI = 1. 004 - 1. 277, P = 0. 048） and （ OR = 1. 663,95 % CI = 1. 406- 1. 968,P=0. 001）,and the patients with rs3743073G variant alleles （TG and GG） were associated with significantly in- creased risk of lung cancer （OR= 1. 290,95 % CI= 1. 150 - 1. 447, P = 0. 001）. Further analysis showed that the increase risk of lung cancer in patients with rs3743073G variant alleles was obvious in patients with age older than 60, male,smok ing （P%0.05）. CONCLUSION： The CHRNA3 gene rs3743073G variant genotype can significantly increase the risk of lung cancer, especially in the patients with age older than 60, male, smoking.