| 巨噬细胞抑制因子-1单克隆抗体的研制及血清检测系统的建立 |
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| 引用本文： | 王小兵,李艳芬,田海梅,李茉,付超,程冬婉,常青云,刘珊,齐军,张伟.巨噬细胞抑制因子-1单克隆抗体的研制及血清检测系统的建立[J].癌症进展,2011,9(4):361-366. |
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| 作者姓名： | 王小兵 李艳芬 田海梅 李茉 付超 程冬婉 常青云 刘珊 齐军 张伟 |
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| 作者单位： | 中国医学科学院,北京协和医学院,肿瘤医院生物检测中心,北京,100021;中国医学科学院,北京协和医学院,肿瘤医院检验科,北京,100021 |
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| 基金项目： | 国家高技术研究发展863计划资助项目 |
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| 摘 要： | 目的 研制巨噬细胞抑制因子-1(MIC-1)单克隆抗体并建立MIC-1血清检测方法.方法 应用自主研制的MIC-1抗原免疫BALB/C雌鼠,通过杂交瘤技术获得分泌抗MIC-1单克隆抗体的杂交瘤细胞株,通过ELISA方法构建MIC-1血清检测体系并对性能进行鉴定.结果 成功获得了32株抗MIC-1单克隆抗体,选用其中2株...
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| 关 键 词： | 巨噬细胞抑制因子-1 肿瘤标志物 单克隆抗体 诊断试剂 |
Development of monoclonal antibody and establishment of detecting system for macrophage inhibitory cytokine-1 |
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| Wang Xiaobing,Li Yanfen,Tian Haimei,Li Mo,Fu Chao,Cheng Dongwan,Chang Qingyun,Liu Shan,Qi Jun,Zhang Wei.Development of monoclonal antibody and establishment of detecting system for macrophage inhibitory cytokine-1[J].Oncology Progress,2011,9(4):361-366. |
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| Authors: | Wang Xiaobing Li Yanfen Tian Haimei Li Mo Fu Chao Cheng Dongwan Chang Qingyun Liu Shan Qi Jun Zhang Wei |
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| Institution: | Wang Xiaobing1 Li Yanfen1 Tian Haimei1 Li Mo1 Fu Chao2 Cheng Dongwan1 Chang Qingyun1 Liu Shan1 Qi Jun2 Zhang Wei1 1 Detection Center of Tumor Biology,2Clinical Laboratory,Cancer Institute & Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100021,China |
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| Abstract: | Objective To develop macrophage inhibitory cytokine-1(MIC-1)monoclonal antibody and the test method for serum MIC-1.Methods BALB/C female mice was immuned by MIC-1 antigen.Then hybridoma cell lines secreting monoclonal antibodies against MIC-1 were obtained by hybridoma technology.ELISA serum test method was constructed and performance was evaluated.Results Totally 32 monoclonal antibodies against MIC-1 were successfully obtained.Two hihg-affinity antiboclies were chosen to establish MIC-1 serum rapid detec... |
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| Keywords: | macrophage inhibitory cytokine-1 tumor marker monoclonal antibody diagnostic kit |
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