应用组织芯片法检测EGFR、COX-2在肺癌中的表达及生物学意义

背景与目的研究表明表皮生长因子受体(epidermal growth factor receptor,EGFR)和环氧合酶-2(cy-clooxygenase-2,COX-2)在多种实体瘤中存在高表达,并且可以通过相应的信号通路调节肿瘤的生长、侵袭和转移。本研究旨在探讨EGFR和COX-2在人类肺癌组织中表达的生物学意义及相互之间的关系。方法应用组织芯片技术结合免疫组织化学SP法检测89例原发肺癌、12例淋巴结转移性肺癌、12例癌前病变(不典型腺瘤样增生)和10例正常肺组织中EGFR、COX-2蛋白的表达情况。结果EGFR在肺癌组、癌前病变组、淋巴结转移性肺癌组中的阳性表达率分别为59.6%(53/89)、41.7%(5/12)和66.7%(8/12),COX-2在上述三组中的阳性表达率分别为52.8%(47/89)、41.7%(5/12)和66.7%(8/12),均较正常组明显升高(P<0.05)。EGFR和COX-2的表达与肺癌的组织学类型、临床分期和淋巴结转移有关(P<0.05),而与组织学分级、性别、年龄无关(P>0.05)。COX-2的表达还与肺癌的大体类型有关(P<0.05)。EGFR和COX-2...

Detection of EGFR and COX-2 Expression by Immunohistochemical Method on a Tissue Microarray Section in Lung Cancer and Biological Significance

Background and objective Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2),which can regulate growth,invasion and metastasis of tumor through relevant signaling pathway,have been detected in a variety of solid tumors.The aim of this study is to investigate the biological significance of EGFR and COX-2 expression in lung cancer and the relationship between them.Methods The expression of EGFR and COX-2 was detected in 89 primary lung cancer tissues,12 premaliganant lesions,12 lymph node met...