miR-26b Mimic Inhibits Glioma Proliferation In Vitro and In Vivo Suppressing COX-2 Expression |
| |
Authors: | Zheng-Gang Chen Chuan-Yi Zheng Wang-Qing Cai Da-Wei Li Fu-Yue Ye Jian Zhou Ran Wu Kun Yang |
| |
Institution: | * Department of Neurosurgery, The First Affiliated Hospital of Hainan Medical College,
Haikou, Hainan, P.R. China† Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University,
Guangzhou, Guangdong, P.R. China |
| |
Abstract: | Glioma is the most common malignant tumor of the nervous system. Studies have shown the microRNA-26b
(miR-26b)/cyclooxygenase-2 (COX-2) axis in the development and progression in many tumor cells. Our study
aims to investigate the effect and mechanism of the miR-26b/COX-2 axis in glioma. Decreased expression of
miR-26b with increased levels of COX-2 was found in glioma tissues compared with matched normal tissues.
A strong negative correlation was observed between the level of miR-26b and COX-2 in 30 glioma tissues. The
miR-26b was then overexpressed by transfecting a miR-26b mimic into U-373 cells. The invasive cell number
and wound closing rate were reduced in U-373 cells transfected with miR-26b mimic. In addition, COX-2
siRNA enhanced the effect of miR-26b mimic in suppressing the expression of p-ERK1 and p-JNK. Finally,
the in vivo experiment revealed that miR-26b mimic transfection strongly reduced the tumor growth, tumor
volume, and expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Taken together, our research
indicated a miR-26b/COX-2/ERK/JNK axis in regulating the motility of glioma in vitro and in vivo, providing
a new sight for the treatment of glioma. |
| |
Keywords: | MicroRNA 26b (miR-26b) Glioma Cyclooxygenase 2 (COX-2) Invasion Migration Extracellular signal-regulated kinase (ERK) |
|
| 点击此处可从《Oncology research》浏览原始摘要信息 |
| 点击此处可从《Oncology research》下载免费的PDF全文 |
|