Knockdown of Long Noncoding RNA CAT104 Inhibits the Proliferation,Migration, and Invasion of Human Osteosarcoma Cells by Regulating MicroRNA-381 |
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Authors: | Bo Xia Lei Wang Li Feng Baofang Tian Yuanjie Tan Baoyin Du |
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Institution: | * Department of Emergency Trauma Surgery, Jining No. 1 People’s Hospital, Jining, Shandong, P.R. China† Second Department of Orthopedics, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,
Jinan, Shandong, P.R. China‡ Department of Cardiology, Weihai Hospital of Traditional Chinese Medicine, Weihai, Shandong, P.R. China |
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Abstract: | Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. This study
aimed to explore the effects of long noncoding RNA CAT104 and microRNA-381 (miR-381) on osteosarcoma
cell proliferation, migration, invasion, and apoptosis, as well as the underlying potential mechanism. We found
that CAT104 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of CAT104 significantly inhibited OS-732 cell proliferation, migration, and invasion, but promoted cell apoptosis. CAT104
regulated the expression of miR-381, and miR-381 participated in the effects of CAT104 on OS-732 cells. Zinc
finger E-box-binding homeobox 1 (ZEB1) was a direct target gene of miR-381, which was involved in the
regulatory roles of miR-381 in OS-732 cell proliferation, migration, invasion, and apoptosis, as well as c-Jun
N-terminal kinase (JNK) and Wnt/ -catenin pathways. In conclusion, our research verified that suppression of
CAT104 exerted significant inhibitory effects on osteosarcoma cell proliferation, migration, and invasion by
regulating the expression of miR-381 and downstream ZEB1, as well as JNK and Wnt/ -catenin pathways. |
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Keywords: | Osteosarcoma Long noncoding RNA CAT104 MicroRNA-381 JNK pathway Zinc-finger E-box-binding homeobox 1 (ZEB1) Wnt/b-catenin pathway |
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