HPV感染与非小细胞肺癌的相关性

背景与目的：人乳头瘤病毒（HPV）是宫颈癌的主要病因，但近来发现在非小细胞肺癌（NSCLC）中能检测到HPV，两者间的关系已经引起了人们的重视。本研究探讨人乳头瘤病毒（HPV）16、18型感染与非小细胞肺癌（NSCLC）是否存在相关性及其意义。方法：应用PCR、免疫组化和TUNEL分别检测76例NSCLC及13例肺良性病变中HPV16、18型DNA及其原癌蛋白E6 E7、端粒酶hTERT、P53、MDM2、桩蛋白（paxillin）和细胞凋亡的表达。结果：NSCLC中HPV16、18型总阳性率为40．8％（31／76），而肺良性病变组阳性率为7．7％（1／13），差异有显著性（P〈0．05）。NSCLC中HPV阳性组与阴性组之间细胞凋亡、P53、MDM2、paxillin的表达均有显著性差异（P〈0．05），而端粒酶hTERT无显著性差异。HPV感染与组织学类型及淋巴结转移无关，而与组织分化程度有显著性差异（P〈0．05）。结论：HPV16、18型感染在NSCLC发生中可能有病因学意义，其致癌机制可能与细胞凋亡、P53、MDM2及paxillin表达变化有关。

A study on the relationship between HPV infection and non-small-cell lung cancer

Background and purpose:Human papillomavirus(HPV) is the main factor for cancer of the cervix,however HPV can be detected in non-small-cell lung cancer(NSCLC) recently.The relationship between them is notable.We investigated whether there was a significant relationship between HPV16,18 infection and non-small-cell lung cancer. Methods:PCR, immunohistochemistry and TUNEL were applied to detect HPV16,18 DNA and oncoprotein E6E7, paxillin, telomerase hTERT, P53, MDM2 and cell apoptosis expression in 76 NSCLC and 13 benign lung specimens, respectively.Results:The positive rates of HPV16, 18 DNA in NSCLC and lenign groups were 40.8%(31/76) and 7.7%(1/13), respectively. There was no relationship between HPV infection and clinicopathological factors of NSCLC including histoclassification and lymph node metastasis except for degree of differentiation (P<0.05). There was statistical significance with regard to cell apoptosis, P53, MDM2 and paxillin expression between HPV positive and negative group in NSCLC except telomerase hTERT (P<0.05).Conclusions:HPV16,18 infection might have etiologic significance for NSCLC, the expression changes of cell apoptosis, P53, MDM2 and paxillin might be one of the carcinogenesis mechanisms.