宫颈癌组织的TNFAIP3和TNFAIP8水平及临床意义

目的探讨宫颈癌组织的肿瘤坏死因子α诱导蛋白(TNFAIP)3和TNFAIP8水平及临床意义。方法收集2015年9月至2018年9月的宫颈癌组织117例、正常宫颈组织49例和宫颈上皮内瘤变(CIN)组织92例,采用实时定量PCR(QPCR)检测上述组织的TNFAIP3和TNFAIP8水平,比较不同宫颈组织的两者水平差异,分析两者与宫颈癌临床病理特征的关系,通过Kaplan-Meier Plotter数据库在线分析TNFAIP3和TNFAIP8与宫颈癌预后的关系。结果49例正常宫颈组织、92例CIN组织和117例宫颈癌组织的TNFAIP3水平依次为1.016±0.062、2.070±0.120和4.521±0.209,TNFAIP8水平依次为1.051±0.092、2.618±0.156和5.240±0.199,宫颈癌组织的TNFAIP3、TNFAIP8水平均高于正常宫颈组织和CIN组织,而CIN组织的两指标水平亦高于正常宫颈组织,差异有统计学意义(P<0.05)。Pearson相关分析显示宫颈癌组织中,TNFAIP3和TNFAIP8水平呈正相关(r=0.617,P<0.001)。TNFAIP3水平与年龄、病理类型、淋巴结转移和月经状态无关(P>0.05),而与肿瘤直径、分化程度、FIGO分期和浸润深度有关(P<0.05)。TNFAIP8水平与年龄、肿瘤直径、病理类型和月经状态无关(P>0.05),而与分化程度、FIGO分期、浸润深度和淋巴结转移有关(P<0.05)。宫颈癌组织TNFAIP3水平与总生存期(OS)有关,而与无复发生存期(RFS)无关,其中低水平者的中位OS为45.73个月,长于高水平者的21.97个月(HR=1.83,95%CI:1.15~2.92),TNFAIP8水平与RFS和OS均无关(P>0.05)。结论宫颈癌组织的TNFAIP3、TNFAIP8水平升高,两者水平有助于判断宫颈癌的分化程度、FIGO分期和浸润深度,TNFAIP3水平还与预后有关,两者可能共同促进了宫颈癌的病情进展。

Level and clinical significance of TNFAIP3 and TNFAIP8 in cervical cancer tissues

Objective To explore the level and clinical significance of tumor necrosis factor-alpha-induced protein(TNFAIP)3 and TNFAIP8 in cervical cancer tissues.Methods From September 2015 to September 2018,117 cases of cervical cancer tissues,49 cases of normal cervical tissues and 92 cases of cervical intraepithelial neoplasia(CIN)tissues were collected.Real-time quantitative PCR(QPCR)was used to detect the levels of TNFAIP3 and TNFAIP8 among the above tissues.The differences of TNFAIP3 and TNFAIP8 in different cervical tissues were compared.The relationship between both factors and the clinicopathological characteristics of cervical cancer was analyzed.Kaplan-Meier Plotter database was used to investigate the relationship between levels of TNFAIP3 and TNFAIP8 and the prognosis of cervical cancer.Results In 49 cases of normal cervical tissues,92 cases of CIN tissues and 117 cases of cervical cancer tissues,levels of TNFAIP3 were 1.016±0.062,2.070±0.120 and 4.521±0.209,and levels of TNFAIP8 were 1.051±0.092,2.618±0.156 and 5.240±0.199,respectively.Levels of TNFAIP3 and TNFAIP8 in cervical cancer tissues were higher than those in normal cervical tissues and CIN tissues,and levels of two factors in CIN tissues were also higher than those in normal cervical tissues(P<0.05).Pearson correlation analysis showed that there was a positive correlation between TNFAIP3 and TNFAIP8(r=0.617,P<0.001).TNFAIP3 level was not related to age,pathological type,lymph node metastasis and menstrual state(P>0.05),but related to tumor diameter,differentiation degree,FIGO stage and infiltration depth(P<0.05).TNFAIP8 level was not related to age,tumor diameter,pathological type and menstrual state(P>0.05),but related to differentiation degree,FIGO stage,infiltration depth and lymph node metastasis(P<0.05).TNFAIP3 level in cervical cancer tissues was related to overall survival(OS),but not to relapse free survival(RFS).The median OS of patients with low-level TNFAIP3 was 45.73 months,longer than 21.97 months of patients with high-level TNFAIP3(HR=1.83,95%CI:1.15-2.92).TNFAIP8 level was not related to RFS and OS(P>0.05).Conclusion Levels of TNFAIP3 and TNFAIP8 in cervical cancer tissues are increased,which are helpful to judge the degree of differentiation,FIGO stage and infiltration depth of cervical cancer.The level of TNFAIP3 is also related to prognosis.Both levels of TNFAIP3 and TNFAIP8 may jointly promote the progress of cervical cancer.