ERCC1在恶性肿瘤患者外周血和肿瘤组织中表达的相关性研究

目的 探讨核苷酸切除修复交叉互补基因1（ERCC1）在肺癌、结肠癌、胃癌、乳腺癌患者外周血和肿瘤组织中表达的相关性及其临床意义。方法 采用实时荧光定量PCR（RT-qPCR）技术检测230例肺癌、160例结肠癌、125例胃癌、100例乳腺癌患者的肿瘤石蜡标本及其对应的血液样本中ERCC1 mRNA的表达水平。结果 ERCC1 mRNA在4种肿瘤的癌组织和外周血中的表达差异均无统计学意义（P＞0.05）。4种肿瘤的癌组织中ERCC1 mRNA的相对表达量高于相对应外周血中的表达（P＜0.05），且肿瘤组织与外周血ERCC1 mRNA的表达均呈正相关（肺癌：r=0.430，P＜0.001；结肠癌：r=0.553，P＜0.001；胃癌：r=0.583，P＜0.001；乳腺癌：r=0.501，P＜0.001）。肺癌、结肠癌及乳腺癌肿瘤组织和外周血中ERCC1的表达水平与年龄、性别均无关（P＞0.05）。胃癌肿瘤组织和外周血中ERCC1 mRNA的表达水平与年龄无关，而男性患者肿瘤组织中ERCC1的表达高于女性（P＜0.05）。结论 通过检测肺癌、结肠癌、胃癌及乳腺癌患者外周血中ERCC1的表达水平能够在一定程度上间接反映癌组织中ERCC1的表达状况。

Relationship between the expressions of ERCC1 in peripheral blood and tumor tissues of malignancies

ObjectiveTo investigate the correlation between the expression of excision repair cross-complementing gene 1（ERCC1）in paraffin tumor tissue and corresponding blood cells of lung，colon，gastric and breast cancer patients. Methods Real-time quantitative PCR was used to detect ERCC1 mRNA expression of 230 lung cancer，160 colon cancer，125 gastric cancer and 100 breast cancer cases from paraffin tumor tissues and corresponding peripheral venous blood samples. Results There was no significant difference in ERCC1 mRNA expression of tumor tissues and peripheral blood among the four types of cancers（P＞0.05）. The expression of ERCC1 in tumor paraffin tissues of lung，colon，gastric and breast cancer were much higher than that in corresponding peripheral blood（P＜0.05）. There was a positive correlation between the expression of ERCC1 mRNA in peripheral blood and cancer tissues（lung cancer：r=0.430，P＜0.001；colon cancer：r=0.553，P＜0.001；gastric cancer：r=0.583，P＜0.001；breast cancer：r=0.501，P＜0.001）. There was no relation between the ERCC1 expression and the clinical characteristics including age and gender in tumor tissues and peripheral blood of lung, colon and breast cancer. The expression of ERCC1 mRNA in tumor tissue and peripheral blood had no correlation with age in gastric cancer, but it had higher expression in male than in female in tumor tissues. Conclusion The expression levels of ERCC1 mRNA in peripheral blood can indirectly reflect their expression levels in lung，colon，gastric and breast cancer tissues.