人核受体nr5a2和GPC3在胃癌组织中的表达及其临床意义

目的 探讨人核受体nr5a2和磷脂酰肌醇蛋白聚糖3（GPC3）在胃癌组织中的表达水平，并分析两者与胃癌临床病理特征及预后的关系。方法 采用免疫组化SP法检测68例胃癌组织和59例癌旁组织石蜡切片中nr5a2和GPC3的表达情况，以阳性细胞百分比与着色强度乘积值分为阴性表达（＜3分）和阳性表达（≥3分），分析两者不同表达与胃癌临床病理参数（性别、年龄、临床分期、肿瘤大小、Lauren分型、分化程度、浸润深度和淋巴结转移）及预后的关系；同时采用Spearman相关分析胃癌组织中nr5a2和GPC3表达的相关性，Kaplan-Meier法分析两蛋白不同表达水平患者的预后情况。结果 胃癌组织中nr5a2和GPC3的阳性表达率分别为64.7％（44／68）和57.4％（39／68），均高于癌旁组织的32.2％（19／59）和22.0％（13／59），差异均有统计学意义（P＜0.05）；胃癌组织中nr5a2和GPC3的表达呈正相关（r=0.421， P=0.001），且两者表达与临床分期和Lauren分型有关，此外nr5a2与浸润深度有关，而GPC3与分化程度和淋巴结转移有关（P＜0.05）。nr5a2阳性表达者的中位生存期（OS）为22.5个月，低于阴性表达者的32.0个月（P＜0.05）；GPC3阳性表达者的中位OS为26.5个月，与阴性表达者的31.0个月比较，差异无统计学意义（P＞0.05）。结论 nr5a2和GPC3在胃癌组织中均以阳性表达为主，两者均与临床分期、Lauren分型有关，且nr5a2表达与预后有关，提示两者可能在胃癌的发生发展中起重要作用，可用于辅助胃癌的诊断和病情评估。

Expression and clinical significance of nr5A2 and GPC3 in human gastric cancer tissues

Objective To investigate the expression levels of nuclear receptor 5A2（nr5A2）and Glypican-3（GPC3）in gastric cancer tissues and the relationship between them and the clinical pathological characteristics and prognosis of gastric cancer. Methods The expression of GPC3 and nr5A2 in paraffin sections of 68 cases of gastric cancer tissues and 59 cases of para carcinoma tissues were detected by immunohistochemical SP method. According to product value of the percentage of positive cells and intensity of staining， the expression levels were divided into negative expression（＜3） and positive expression（≥3）. We analyzed the relationship between the different expression and clinical pathological parameters（gender， age， clinical stage， tumor size， Lauren type， differentiation degree， invasion depth and lymph node metastasis）and prognosis of gastric cancer. Spearman correlation analysis was used to analyze the expression of GPC3 and nr5A2 in gastric cancer tissues， and the prognosis of patients with different expression of the both proteins was analyzed by Kaplan Meier method. Results The positive expression rates of nr5A2 and GPC3 in gastric cancer tissues were 64.7％（44／68）and 57.4％（39／68）， higher than 32.2％（19／59）and 22.0％（13／59）of the adjacent tissues with statistical significance（P＜0.05）. The expression of nr5A2 was positively correlated with GPC3 in gastric cancer tissues（r=0.421， P=0.001）. The expression of the both proteins was related to clinical stage and Lauren typing. In addition， the expression of nr5A2 was related to the depth of invasion， and the expression of GPC3 was related to the degree of differentiation and lymph node metastasis（P＜0.05）. The median survival time（OS）of nr5A2 positive expression patients was 22.5 months， lower than 32.0 months of nr5A2 negative expression patients（P＜0.05）. The difference was not statistically significant between different expression levels of GPC3. Conclusion Both nr5A2 and GPC3 are positive expression in gastric cancer tissues， which is related to clinical stage and Lauren typing. nr5A2 expression is related to prognosis. Both proteins may play an important role in the development of gastric cancer， which can be used in the diagnosis and evaluation of gastric cancer.