鼻咽癌组织中c-Fos和Cyclin D1蛋白表达的检测及意义

目的:检测鼻咽癌组织中c-Fos、Cyclin D1的表达,并探讨其与鼻咽癌临床病理特征及预后的关系.方法:收集1996年4月至1998年6月在中山大学肿瘤防治中心鼻咽癌科行鼻咽活检的鼻咽癌患者的标本55份.采用免疫组化LSAB法对55例鼻咽癌组织中c-Fos、Cyclin D1的表达进行检测,用统计学软件SPSS10.0进行数据处理.结果:c-Fos及CyclinD1阳性表达率为分别为94.5%和78.2%,阳性表达部位均为细胞核;两种蛋白的表达与鼻咽癌92'临床分期、T分期、N分期、年龄、性别皆无显著相关性;c--Fos的表达时鼻咽癌患者的预后无显著性影响:Cyclin D1阴性患者的总生存期(OS)、至疾病进展时间(TTP)显著低于阳性患者.COX多因素相关分析年龄＞50岁、Cyclin D1阴性是不良预后因素.结论:鼻咽癌组织中存在c-Fos、Cyclin D1高表达,Cyclin D1阴性是独立的不良预后因素.

The Significance of c-Fos,and Cyclin D1 Expression in Nasopharyngeal Carcinoma

Objective: To detect the expression of c-Fos, and Cyclin D1 in Nasopharyngeal Carcinoma (NPC) and to investigate the associatin between the expression, clinicopathological characteristics and the prognosis of NPC patients. Methods: Archival formalin fixed, paraffin embedded specimens from 55 primary NPC patients, who had biopsy in Cancer center, Sun Yat-sen University during April in 1996 to June in 1998, were investigated. Immunohistochemistry LSAB method was adopted to detect the expression of c-Fos, Cyclin D1. The statistical analysis was performed using SPSS statistical software package(10.0). Results: Positive staining for c-Fos was observed in cancer cell nucleus in 52 of 55 cases(94.5%). The expression of Cyclin D1 was positive in 47 of 55 cases(78.2%) and the staining site of Cyclin D1 was also in cancer cell nucleus. No significant association was observed among c-Fos, Cyclin D1 status and clinical stage, T stage, N stage, the sex and age. There was no statistically significant correlation between the c-Fos status and prognosis. Positive Cyclin D1 expression correlated with better OS and TTP. No significant correlation was found between Cyclin D1 status and DFS. On multivariate analysis, the age was more than 50 years and negative Cyclin D1 was an independent poor prognostic factors for NPC. Conclusions: The Overexpression of c-Fos and Cyclin D1 has been observed in NPC and the negative Cyclin D1 is an independent poor prognostic factor for NPC.