胃癌组织中miR-222与TIMP3的表达及临床意义

  目的  探讨miR-222(Homo sapiens miR-222)与基质金属蛋白酶抑制因子3(Tissue inhibitor of metalloproteinases-3, TIMP3)在胃癌组织中的表达及其临床意义。  方法  分别运用原位杂交、免疫组化检测胃癌组织及癌旁组织中miR-222与TIMP3的表达水平, 分析miR-222、TIMP3之间的相关性以及与胃癌临床病理参数的关系。  结果  原位杂交检测显示, 在62例胃癌组织中miR-222阳性表达率为86.67%, 与癌旁对照组22.58%比较, 差异有统计学意义(P < 0.01)。免疫组化结果显示, 在62例胃癌组织中TIMP3阳性表达率为19.35%, 与癌旁对照组75.81%比较, 差异有统计学意义(P < 0.01)。相关性分析表明, miR-222的表达与TIMP3的表达呈负相关(P < 0.05);光密度值检测发现, miR-222的表达随着胃癌临床分期和浸润深度的演进而增加, 且胃癌中有淋巴结转移的miR-222的表达显著高于无淋巴结转移组(P < 0.01);TIMP3的表达随着胃癌临床分期和浸润深度的演进而下调, 胃癌中有淋巴结转移的TIMP3的表达均显著低于无淋巴结转移组(P < 0.01)。  结论  在胃癌组织中高表达的miR-222和TIMP3蛋白低表达可能是胃黏膜恶性转变以及胃癌发生浸润转移的重要生物学标志, 检测miR-222和TIMP3对预测结肠癌浸润转移有重要意义。 

Expression and Clinical Significance of MiR-222 and TIMP3 in Gastric Cancer

  Objective   To investigates the expression of miR-222 and TIMP3 in gastric cancer, as well as their clinical significance.   Methods   In situ hybridization and immunohistochemistry were used to detect the expression of miR-222 and TIMP3 in gastric cancer specimens, respectively.The correlations among the miR-222, TIMP3 protein, and clinicopathological parameters of gastric cancer were analyzed.   Results   In situ hybridization showed that the positive expression rate of miR-222 was 86.67 % and 22.58%in gastric cancer and the adjacent tissue, respectively.In the assay of immunohistochemistry, the positive expression rate of TIMP3 was 19.35 % and 75.81 % in gastric cancer and the adjacent tissue, respectively.Significant differences(P < 0.01) were found between the two aforementioned groups.The correlation analysis indicated that the expression of miR-222 had a close negative correlation with that of TIMP3 in gastric cancer(P < 0.01).The up-regulation of miR-222 expression was associated with an advanced clinical stage, infiltrating depth, and lymph node metastasis in the cancer(P < 0.01).Conversely, the down-regulation of the TIMP3 expression was associated with an advanced clinical stage, infiltrating depth, and lymph node metastasis in the cancer(P < 0.01).   Conclusion   The high expression of miR-222 and the low expression of the TIMP3 protein may be important biological markers for malignant transformation in the invasion and metastasis of gastric cancer.The detection of miR-222 and TIMP3 expression in gastric cancer is significantly important for improving the prediction of invasion and metastasis.