肝细胞肝癌组织中Bcl-2核表达及MMP-2和MMP-9表达与术后复发转移及预后的关系

  目的  探讨抗凋亡蛋白Bcl-2、基质金属蛋白酶-2(MMP-2)和MMP-9在肝细胞肝癌中表达的关联性, 及其与复发、转移和预后的关系。  方法  以石蜡包埋组织切片, 免疫组织化学SP法染色检测97例肝细胞肝癌, 分别在复发与转移组49例和非复发与转移组48例中分析Bc1-2核表达和MMP-2、MMP-9表达的情况。  结果  复发与转移组Bcl-2核表达高于无复发与转移组(x2=7.912, P=0.005), 复发转移组MMP-9表达高于无复发转移组(x2=6.545, P=0.011), Bcl-2核表达阳性细胞率与MMP-9阳性细胞率存在相关性, 而与MMP-2无关Bcl-2核表达组患者生存时间较短, 差异具有统计学意义。  结论  Bcl-2核表达阳性患者更易出现复发与转移, 且与MMP-9过度表达存在关联性, 表明Bcl-2入核可能对肿瘤转移的相关生物学功能具有调节作用, 可作为评价HCC复发与转移、不良生存预后的候选临床标志。 

Relationship of Bcl-2 Expression with MMP2 and MMP9 Expression in Hepatocellular Carcinoma,and Their Correlation with Recurrence, Metastasis, and Prognosis

  Objective  To determine the correlation of the expression of the anti-apoptotic protein Bcl-2 with the expression of matrix metalloproteinases MMP-2 and MMP-9 in human hepatocellular carcinoma, and their relationship with recurrence, metastasis, and prognosis.  Methods  Paraffin-embedded tissue sections of 97 hepatocellular carcinoma cases were subjected to SP immunohistochemical staining.The nuclear expression of Bcl-2 as well as the expression of MMP-2 and MMP-9 were detected.A total of 49 cases had recurrence and metastasis, and the remaining 48 did not.  Results  The nuclear expression of Bcl-2 was higher in cases with recurrence and/or metastasis(t = -2.488, P = 0.015).Similarly, MMP-2 and MMP-9 expression were higher in cases with recurrence and/or metastasis(Z = -2.623, P = 0.009;Z = -2.028, P = 0.043).The nuclear expression of Bcl-2 was correlated with the expression of MMP-9, but not with that of MMP-2.Patients with positive nuclear expression of Bcl-2 had shorter survival time, and the difference was significant.  Conclusion  The nuclear expression of Bcl-2 was significantly correlated with metastasis and MMP-9 expression. Bcl-2 relocation into the nucleus may regulate metastasis-related proteins, which can be used as a clinical marker for evaluating recurrence, metastasis, and prognosis.