宫颈病变Ki67和P53表达的定量分析及其临床意义

目的定量探讨Ki67和P53在子宫颈鳞状细胞癌（SCC）、宫颈上皮内瘤变（CIN）及慢性炎症中的表达特点、变化规律及其临床意义。方法用免疫组化和图像定量分析技术检测11例宫颈癌、27例宫颈上皮内瘤变（其中CINⅠ10例，CINⅡ6例，CINⅢ11例）及7例子宫颈慢性炎症（CC—itis）的石蜡标本中Ki67和P53表达的阳性单位。结果Ki67和P53在SCC、CIN及CC—itis组织中表达的阳性单位（PU）差异具有显著性（F=26．447，F=9．564，P均〈0．001），SCC、CIN病变中Ki67和P53表达的阳性单位明显高于CC．itis，差异具有显著性（P均〈0．001）。CIN病变中Ki67的阳性单位具有随CIN分级增高而增大的趋势（单侧检验P〈0．05）。CINⅢ组中Ki67表达的阳性单位明显高于CINⅠ组，差异有统计学意义（P=0．023）；P53的阳性单位在CIN不同级别的比较中差异具有显著性（P=0．01），CINⅡ组中P53的阳性单位明显高于CINⅠ组，差异有统计学意义（P=0．003）。宫颈病变需手术组的Ki67和P53的阳性单位均明显高于保守治疗组，差异具有统计学意义（P均〈0．001）。结论Ki67和P53的表达强度与宫颈慢性炎症、宫颈上皮内瘤变及宫颈鳞状细胞癌有关，两者在组织中表达的阳性单位对于区分宫颈炎性病变和上皮内瘤变，区分低级别和高级别上皮内瘤变，包括CINI和CINⅡ的区分，以及对于治疗方案的拟定均具有重要价值；此外Ki67表达的阳性单位有助于CIN分级。

Quantitative analysis of the expression and clinical significance of P53 and Ki67 in cervical lesions

Objective To explore the quantitative expression and clinical significance of Ki67 and P53 in cervical squamous cell carcinoma （SCC） , cervical （CC-itis）. Methods The expressions of Ki67 and in paraffin-embedded specimens including 11 cases intraepithelial neoplasia （CIN） and chronic cervicitis p53 were detected by immunohistochemical technique of cervical cancer, 27 cases of cervical intraepithelial neoplasia （CIN） （including 10 case of CIN I , 6 CIN II and 11 CIN III ） , and 7 cases of chronic cervicitis. The expression features and varying patterns of Ki67 and P53 were assessed by quantitative analysis Results Positive unit （PU） values of Ki67 and p53 in SCC and CIN were significantly higher than those in CC-itis （F = 26.447, F = 9. 564, P all 〈 0. 001 ）. PU value of Ki67 increased with the grade of CIN （ One side test P 〈 0.05）. PU value of Ki67 of the CIN III group was significantly higher than that of the CIN1 group （P =0. 023）. There was a significant difference of PU values of P53 among the CIN grades （P = 0.01 ）. PU value of P53 of the CIN 11 group was significantly higher than that of the CIN I group （P = 0. 003）. PU value of Ki67 and P53 of the surgery-requiring group was significantly higher than that of the non-operative group （P all 〈 0. 001 ）. Conclusions The expressions of Ki67 and P53 are associat- ed with CIN and SCC. Detection of P53 flammatory lesions, low grade and high choose treatment method. In addition, and Ki67 expression is helpful to grade CIN, as well as CIN I and distinguish between CIN and in- CIN II ~ They are also helpful to Ki67 may be an indicator in CIN grading