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| 鞘鞍醇激酶1对肝癌细胞凋亡特性的影响 | |
| 引用本文： | 陈海英,高艳景,刘慧亚,陈雨信,姜大磊,袁勇.鞘鞍醇激酶1对肝癌细胞凋亡特性的影响[J].中国现代普通外科进展,2011,14(1):6-8,16. |
| 作者姓名： | 陈海英 高艳景 刘慧亚 陈雨信 姜大磊 袁勇 |
| 作者单位： | 1. 山东大学齐鲁医院消化内科山东,济南,250012;教育部和卫生部心血管重构和功能研究重点实验室,山东,济南,250012 2. 山东大学齐鲁医院消化内科山东,济南,250012 3. 山东大学齐鲁医院肝胆外科,山东,济南,250012 4. 青岛市立医院消化内科,山东,青岛,260000 5. 青岛大学医学院附属医院医政部,山东,青岛,266000 |
| 基金项目： | 山东省自然科学基金资助课题(Y2006C53) |
| 摘 要： | 目的：研究鞘鞍醇激酶1（SPK1）对人肝癌耐药细胞株BEL-FU凋亡特性的影响。方法：扩增并纯化携带野生型（Ad-SPK1WT）和SiRNASPK1（Ad-H1-SPK1）的重组腺病毒,以TCID50方法（I-U/ml）和快速CPE法测定病毒感染滴度;以腺病毒为载体将SPK1基因导入肝癌细胞BEL-FU;分别以酶活性检测试剂盒、MTT法检测SPK酶活性及SPK1对肝癌细胞凋亡特性的影响。结果：获得高滴度的Ad-SPK1WT腺病毒和Ad-H1-SPK1腺病毒;携带Ad-SPK1WT基因的BEL-FU细胞中SPK1酶活性增强并抑制DMS（N,N,-二甲基鞘氨醇）对BEL-FU的细胞毒作用,抑制肿瘤细胞凋亡;携带Ad-H1-SPK1基因的BEL-FU细胞中SPK1酶活性降低并增强DMS（N,N,-二甲基鞘氨醇）对BEL-FU的细胞毒作用,促进肿瘤细胞凋亡。结论：阻断SPK1作用可能成为肝癌治疗的新途径。 |
| 关 键 词： | 鞘鞍醇激酶 肝肿瘤 细胞凋亡 |
Effect of sphingosine kinase on the apoptosis of human hepatocellular carcinoma cell |
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| CHEN Hai-ying,GAO Yan-jing,LIU Hui-ya,CHEN Yu-xin,JIANG Da-lei,YUAN Yong.Effect of sphingosine kinase on the apoptosis of human hepatocellular carcinoma cell[J].Chinese Journal of Current Advances in General Surgery,2011,14(1):6-8,16. | |
| Authors: | CHEN Hai-ying GAO Yan-jing LIU Hui-ya CHEN Yu-xin JIANG Da-lei YUAN Yong |
| Institution: | CHEN Hai-ying1,2,GAO Yan-jing1,LIU Hui-ya1,CHEN Yu-xin 3,JIANG Da-lei4,YUAN Yong5 1Department of Gastroenterology,3Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University(Jinan 250012,China) 2Key Laboratory of Cardiovascular Remodeling and Function Research,Chinese Ministry of Education and Chinese Ministry of Public Health(Jinan 250012,China) 4Department of Gastroenterology,Qingdao Municipal Hospital(Qingdao 260000,China) 5Medical Post Office Department,Subsidiary hospital of Qingdao Unive... |
| Abstract: | Objective: To investigate the role of sphingosine kinase 1(SPK1) on the apoptosis of human hepatocellular carcinoma cell line BEL-FU.Methods:The adenovirus carrying the wild type SPK1 gene(SPK1WT) and SiRNASPK1(Ad-H1-SPK1) were constructed respectively;viral infection titer was determined by TCID50 and CPE.Methods: Cellular SPK enzyme activity was assayed;the effect of SPK1 on the apoptosis of BEL-FU was evaluated by MTT.Results: Readenoviruses were amplified and purified;SPK enzyme activity assay suggested... |
| Keywords: | Sphingosine kinas·Live neoplasms·Apoptosis |
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