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| 辛伐他汀对人脐静脉内皮细胞分泌IL-6和IL-8的影响 | |
| 引用本文： | JI Ran,季冉,张立平,邓景惕,田铧,窦姗姗,郝作斌,徐禾介.辛伐他汀对人脐静脉内皮细胞分泌IL-6和IL-8的影响[J].中国现代普通外科进展,2008,11(3):242-244. |
| 作者姓名： | JI Ran 季冉 张立平 邓景惕 田铧 窦姗姗 郝作斌 徐禾介 |
| 作者单位： | 1. 山东大学医学院,解剖学教研室,山东,济南,250012 2. 济南市第三人民医院,普外科,山东,济南,250101 3. 济南市中心医院,普通外科,山东,济南,250014 |
| 摘 要： | 目的：研究辛伐他汀对人脐静脉内皮细胞分泌IL-6和IL-8的影响。方法：辛伐他汀（5μg／mL，10μg/mL）分别预处理细胞8h后加入100ng／mL的脂多糖（ipopolysaccharides，LPS）共同孵育，用ELISA方法检测不同时间点（1、12和24h）JL-6和IL-8含量变化。结果：空白对照组细胞在不施加任何刺激的情况下可以分泌少量IL-6和JL-8，并随时间的延长分泌量不断增加；LPS刺激组IL-6和IL-8分泌量比对9鼐组明显增加．差异有统计学意义（P〈0．05）；经过辛伐他汀预处理组IL-6和IL-8分泌量明显低于LPS单独刺激（P〈0．01）。结论：辛伐他汀具有抑制LPS刺激血管内皮细胞分泌炎症因子JL-6和JL-8的作用。 |
| 关 键 词： | 动脉粥样硬化 辛伐他汀 脂多糖 人脐静脉内皮细胞 |
Effect of simvastatin on the secretion of IL-6 and IL-8 in human umbilical vein endothelial cells |
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| JI Ran.Effect of simvastatin on the secretion of IL-6 and IL-8 in human umbilical vein endothelial cells[J].Chinese Journal of Current Advances in General Surgery,2008,11(3):242-244. | |
| Authors: | JI Ran |
| Institution: | JI Ran, ZHANG Li-ping, DENG Jing-ti, TIAN Hua, DOU Shan-shan, HAO Zuo-bin, XU He-jie (1 Department ofAnatomy, School of Medicine Shandong University (Jinan 250012, China); 2 Department of General Surgery, the Third People＇s Hospital of Jinan (Jinan 250101, Cln＇na );3 Department of General Surgery, Central Hospital of Jinan (Jinan 250013, China)) |
| Abstract: | Objective： To observe the effect of simvastatin on the secretion of IL-6 and IL-8 in human umbilical vein endothelial cells. Method： Human umbilical vein endothelial cells were stimulated by LPS （100 ng/ml）and co-incubated with different concentrations of simvastatin （5 μ g/mL,10 μ g/mL） for 1-24 hours （1 h,12 h and 24 h）. The levels of IL -6 and IL -8 in the cells were measured by ELISA. Result： LPS could increase the production of both IL-6 and IL-8 in umbilical vein endothelial cells significantly. The levels of these cytokines were reduced significantly by simvastatin when compared with blank control group （P〈0.01）. Conclusion： Simvastatin can reduce the levels of IL-6 and IL-8 in the human umbilical vein endothelial cells, which is stimulated by LPS. |
| Keywords: | Atherosclerosis·Simvastatin· Lipopolysaccharide·Human umbilical vein endothelial cell |
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