hi-FGF-2对离体大鼠心缺血/再灌注损伤的拮抗作用

目的 观察高相对分子质量成纤维细胞生长因子-2(hi-FGF-2)对离体大鼠心肌缺血/再灌注损伤的影响.方法 利用Langendorff装置将大鼠心脏给予缺血30 min,再灌注60 min处理.再灌的前10 min经灌流液分别给以生理盐水、hi-FGF-2以及低相对分子质量FGF-2(lo-FGF-2,10μg/10 ml生理盐水).测定左心室收缩压(LVSP)、左心室发展压(LVDP)、左室内压最大变化速率(±dp/dtmax)以及冠脉血流量;采用免疫荧光法检测外源性FGF-2在心肌内的分布和细胞膜上vinculin的变化.结果 与生理盐水组比较,hj-FGF-2和lo-FGF-2两组的心功能包括LVSP、LVDP、±dp/dtmax以及冠脉血流量均显著升高(P＜0.05或0.01);外源性hi-FGF-2或lo-FGF-2在再灌注1 h后广泛分布于心肌.与生理盐水组比较,hj-FGF-2和lo-FGF-2两组的心肌可见密集完整的vinculin荧光染色.结论 hi-FGF-2和lo-FGF-2一样对心肌缺血/再灌注损伤具有拮抗作用.

Antagonistic effects of hi-FGF-2 on myocardial ischemia/reperfusion injury in rats

Objective To investigate the effect of CUG-initiated, high molecular weight fibroblast growth factor-2 (hi-FGF-2) on myocardial ischemia/reperfusion injury in rats. Methods Ex vivo rat hearts were subjected to 30 rain ischemia and 60 min reperfusion. Normal saline, hi-FGF-2 or low molecular weight FGF-2 (lo-FGF-2) (10 μg in 10 ml normal saline) was perfused with perfusate into the heart during the first 10 min of reperfusion. Distribution of exogenous FGF-2, changes of vinculin as well as cardiac mechanic functions were examined. Results Cardiac functions including systolic pressure, dp/dtmax and developed pressure were significantly improved in hi- and lo-FGF-2 groups compared to saline controls (P <0.05 or 0. 01 ). The exogenous hi- or lo-FGF-2 was broadly distributed into the myocardium during 1 h reperfusion. Intensive and intact vinculin staining was seen in both lo- and hi-FGF-2, but not in normal saline, administered hearts. Conclusion hi-FGF-2, like its low molecular weight isoform, is cardioprotective against myocardial ischemia/reperfasion injury.