全生物化组织工程血管的体外构建

目的 探讨组织工程血管的体外构建:脱细胞血管基质的制备.血管平滑肌细胞和血管内皮祖细胞的体外诱导培养和种植方法.方法 处理猪胸主动脉来获得脱细胞血管基质,采用二步种植法先后种植体外培养的平滑肌细胞和内皮细胞.结果 动脉管壁细胞全部脱除,脱细胞基质胶原蛋白含量与新鲜动脉相似,胶原纤维、弹性纤维呈网状排列,无断裂,基质保持完好.脱细胞血管基质的极限应力比新鲜动脉绀织减小20%新鲜动脉:(1.1510±1.2870)×10-2,脱细胞血管基质:(0.9215±1.7000)×10-2,t=34.137,P<0.01].种植的平滑肌细胞和内皮细胞生长良好.结论 平滑肌细胞和内皮细胞种植于脱细胞血管基质后生长良好,可体外构建组织工程血管.

Construction of complete biological tissue engineering blood vessel

Objective To study the methods for construction of complete biological tissue engi-neering blood vessel,mainly including the preparation of acellular vascular matrix(AVM),in vitro culture and seeding of the smooth muscle cells (SMCs) and endothelial cells (Ecs). Methods Trypsin, hyper- tonic solutions and chemical detergent were applied for a multi-step process to carry out the preparation of AVM from pigs. The SMCs and Ecs were seeded successively by two steps. Results The cells were re-moved completely in pig blood vessels, the collagen protein level of acellular matrix was similar to the fresh aorta,and the elastic fibers and collagen fibers were preserved well. The ultimate stress measured in AVM was reduced by 20% as compared with fresh arterial tissue Fresh artery:(1. 1510 ± 1. 2870)× 10-2, AVM:(0.9215 ± 1. 7000)×10-2 ,t = 34. 137 ,P <0.01]. After seeding,the SMCs and the Ecs grew well on the matrix. Conclusion It is feasible to prepare the tissue engineering blood vessel by seeding SMCs and Ecs on the AVM.