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手术/创伤后外周静脉血内毒素的研究
引用本文:徐鹏远,蒋朱明,孙永华.手术/创伤后外周静脉血内毒素的研究[J].中华实验外科杂志,1999,16(4):298-299.
作者姓名:徐鹏远  蒋朱明  孙永华
作者单位:昆明医学院附属第二医院普外科,北京协和医院,北京积水潭医院烧伤科
基金项目:卫生部重点资助,卫生部国际交流中心资助
摘    要:目的探讨健康成人和创伤病人外周静脉血内毒素水平及其与创伤的关系。方法采用偶氮显色鲎试验检测50例健康成人及51例不同手术/创伤病人外周静脉血的内毒素。结果(1)此法检测内毒素可达微量(10-2Eu/ml,n=5),重复性较好(CV=2.29%,n=5)。(2)本法测得健康成人外周静脉血内毒素<0.033Eu/ml,与国际水准一致。(3)病人术后3天内毒素升高(P<0.01)。(4)病人外周静脉血内毒素与创伤程度呈正相关,R2=0.667。结论本法测定血微量内毒素是可行的。外周静脉内毒素水平随创伤程度增加而增加。

关 键 词:手术/创伤  内毒素移位  偶氮显色鲎试验

Study on endotoxin levels in peripheral venous blood after operation/trauma
\ XU Pengyuan,JIANG Zhuming,SUN Yonghua,et al..Study on endotoxin levels in peripheral venous blood after operation/trauma[J].Chinese Journal of Experimental Surgery,1999,16(4):298-299.
Authors:\ XU Pengyuan  JIANG Zhuming  SUN Yonghua  
Institution:\ XU Pengyuan,JIANG Zhuming,SUN Yonghua,et al.\ Department of General Surgery,The Second Affiliated Hospital,Kunming Medical College,Kunming 650101
Abstract:Objective\ To investigate the relationship between the endotoxin level in peripheral venous blood and trauma in the patients with trauma and healthy adults. Methods\ Peripheral venous blood samples were taken from 50 healthy people and 51 patients with various surgical operations/trauma and the endotoxin levels in peripheral venous blood assayed by using Limulum lysate test with chromogenic substrate. Results\ Improved limulus lysate test with chromogenic substrate could determine the trace amount endotoxin (10 -2 Eu/ml, n =5)and had a good reproducible (CV=2.29%, n =5). Endotoxin levels in 50 healthy adults in peripheral venous blood was below 0.0330 Eu/ml. Endotoxin concentration at the 3rd day after operation was obviously increased as compared with that before operation ( P <0.01). Endotoxin levels in peripheral venous blood were correlated with the severity of stress. Conclusion\ This method might be feasible for determining trace amount endotoxin. Endotoxin levels in peripheral venous blood were positively correlated with the severity of stress.
Keywords:Operation/trauma  Endotoxin translocation  Limulus lysate test with chromogenic substrate
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