胰腺癌黏蛋白4基因修饰的树突状细胞疫苗构建及对胰腺癌细胞的免疫效应 |
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引用本文: | 任华建,张烨,卫积书,高文涛,徐泽宽,苗毅. 胰腺癌黏蛋白4基因修饰的树突状细胞疫苗构建及对胰腺癌细胞的免疫效应[J]. 中华实验外科杂志, 2006, 26(1): 830-832. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.005 |
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作者姓名: | 任华建 张烨 卫积书 高文涛 徐泽宽 苗毅 |
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作者单位: | 南京医科大学第一附属医院普外科,210029; |
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基金项目: | 国家自然科学基金资助项目 |
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摘 要: | Objective To obtain the dendritic cells ( DC)-based vaccine modified by adenovirus containing MUC4 gene , and evaluate the anti-tumor efficacy of DC vaccine to pancreatic tumor cells. Meth-ods The mRNA sequence of tumor associated antigen, MUC4, was obtained from NCBI, and MUC4 se-quence was acquired through the restriction enzyme sites and over lap PCR, then subcloned into adenovirus plasmid to create recombinant adenovirus ( rAd-MUC4) . The DCs were infected by rAd-MUC4 virus and then stimulated the lymph cells from the same donor to induce MUC4 specific cytotoxicity T lympbocytes ( CTL) . The efficacy of CTL was analyzed by LDH releasing assay. Elispot was used to detect the IFN-γ release. Results The recombinant adenovirus containing MUC4 ( sv12) gene was obtained. The MUC4-induced CTL could specifically kill the Capan-1 pancreatic tumor cells [ ( 13. 7±6.0)% , ( 21.4± 4. 7)% , (36.1±9. 5)% at ratios of 10: ,20: ,40: ] , higher than MCF-7 and Bxpc-3 cells respectively, P < 0. 05. The spots number of CTL induced by rAd-MUC4 was ( 139.1±23.3) , more than GFP and PBS control group,P<0.05. Conclusion The Muc4 gene modified DC vaccine could induce the proliferation of CTL, which provided a significant cytotoxicity to HLA-matched MUC4 positive tumor cell lines in vitro.
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关 键 词: | 胰腺癌 疫苗 腺病毒载体 树突状细胞 |
Dendritic cells expressing muc4 gene induce cytotoxic T-cell responses in vitro |
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Abstract: | |
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Keywords: | Pancreatic carcinomaVaccineAdenovirus vectorDendritic cell |
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