大肠癌中脆性组氨酸三联体基因和Survivin基因的表达及临床意义

目的检测脆性组氨酸三联体（FHIT）基因和Survivin基因在大肠癌、正常大肠黏膜上皮组织中的表达，探讨FHIT基因和Survivin基因与大肠癌．发生发展及转移的关系，探讨其与大肠癌临床病理特征的关系。方法利用免疫组织化学法对56例大肠癌和33例正常大肠黏膜组织检测FHIT、Survivin基因的表达。结果FHIT蛋白在33例癌旁正常大肠黏膜上皮和56例发癌中的阳性表达率分别为93．9％、48．2％，原发癌中FHIT蛋白的阳性表达率明显低于癌症正常大的黏膜上皮（P〈0，01）。Survivin在56例大肠癌的表达率为48．2％，显著高于33例癌症正常大肠黏膜上皮（P〈0．01）。结论FHIT基因表达的下调与Survivin基因表达的上调可能促进了大肠癌的发生。这样临床上通过检测FHIT、Survivin的基因的表达率对患者的预后有重要的指导意义。

Expression of FHIT and Survivin genes in colorectal carcinoma and their significance

Ojbective To detect the expression of FHIT gene and Survivin gene in colorectal carcinomas and normal colorectal tissues and their correlation with carcinogenesis, development, metastasis and clinicopathological characteristics of colorectal carcinomas. Methods The expression of FHIT and Survivin in 55 cases of colorectal cancers and 33 cases of the adjacent normal colorectal tissues was detected by immunohistochemical SP technique. Results The expression positive rate of FHIT protein in 33 cases of the adjacent normal tissues and 56 cases of primary colorectal carcinomas was 93.9 % and 48.2 % respectively with the difference being significant (P<0.01) .The positive rate of Survivin in 56 cases of primary colorectal carcinomas was 48.2 %, significantly higher than that in 33 cases of the adjacent normal tissues (P<0.01) .Conclusion The low expression of FHIT and high expression of Survivin may promote the development of colorectal carcinoma. The detection of the expression rate of FHIT and Survivin is of an important prognostic value in patients with colorectal carcinomas.