泌尿系统组织工程支架材料胶原蛋白-壳聚糖及聚乙烯醇-丝胶的生物相容性评价

目的评价复合材料胶原蛋白-壳聚糖、聚乙烯醇-丝胶作为泌尿系统组织工程支架的生物相容性和生物安全性。方法采用溶血实验评价胶原蛋白-壳聚糖、聚乙烯醇-丝胶两种复合材料对红细胞功能和代谢的影响;采用皮肤致敏实验评价两种材料是否满足体内植入的要求;采用四甲基偶氮唑盐微量酶反应比色法评价胶原蛋白-壳聚糖、聚乙烯醇-丝胶两种复合材料对泌尿上皮细胞和膀胱平滑肌细胞的生长和增殖的影响。结果胶原蛋白-壳聚糖和聚乙烯醇-丝胶的溶血率分别为1.86%和2.08%(均<5%),两种材料不引起溶血反应。与阴性对照组相比,两种材料皮肤致敏实验的结果均为阴性,没有致敏作用。细胞毒性实验(四甲基偶氮唑盐微量酶反应比色法)显示两种材料细胞毒性均为0级。结论复合生物材料胶原蛋白-壳聚糖、聚乙烯醇丝胶具有良好的生物相容性,有望作为组织工程支架材料,应用于泌尿系统的修复重建。

Biocompatibility Evaluation of Collagen-Chitosan and Polyvinyl Alcohol-Sericin as Tissue Engineered Scaffold in Urinary System

Objective To investigate the biocompatibility of collagen-chitosan and polyvinyl alcohol-sericin as tissue engineered scaffold in urinary system.Methods Hemolytic test was performed to evaluate these materials′（collagen-chitosan and polyvinyl alcohol-sericin） impact on the function and metabolism of erythrocyte.Skin sensitization test was used to investigate the feasibility of these materials as the implantation materials for human body.Cytotoxicity test（MTT assay） was used to evaluate these materials′ influence on the growth and proliferation of cultured cells（urothelial cells and bladder smooth muscle cells）.Results The hemolytic rate of collagen-chitosan and polyvinyl alcohol-sericin was 1.86% and 2.08% respectively（5%）,and no hemolytic effect was observed.Skin sensitization test showed no difference between experimental groups（collagen-chitosan group and polyvinyl alcohol-sericin group） and control group,which indicated that these materials meet the requirements of implantation.The MTT assay showed that cytotoxicity score of collagen-chitosan and polyvinyl alcohol-sericin were both grade 0.Conclusion Both of collagen-chitosan and polyvinyl alcohol-sericin have good biocompatibility and should be safe and promising in urinary reconstruction as tissue engineered scaffold.